T0565
3,3′,5,5′-Tetramethylbenzidine
TMB membrane substrate, chromogenic, liquid
Synonym(s):
TMB membrane substrate
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About This Item
Product Name
3,3′,5,5′-Tetramethylbenzidine (TMB) Liquid Substrate System for Membranes, ready to use solution
Quality Level
form
liquid
storage temp.
2-8°C
General description
Application
- enzyme-linked immune absorbent spot (ELISPOT) assay
- as a substrate for IgG peroxidase
- in the visualization of immunocomplexes
Physical form
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Storage Class
12 - Non Combustible Liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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Articles
The field of proteomics is continually looking for new ways to investigate protein dynamics within complex biological samples. Recently, many researchers have begun to use RNA interference (RNAi) as a method of manipulating protein levels within their samples, but the ability to accurately determine these protein amounts remains a challenge. Fortunately, over the past decade, the field of proteomics has witnessed significant advances in the area of mass spectrometry. These advances, both in instrumentation and methodology, are providing researchers with sensitive assays for both identification and quantification of proteins within complex samples. This discussion will highlight some of these methodologies, namely the use of Multiple Reaction Monitoring (MRM) and Protein-AQUA.
NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.