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T0910

Thioredoxin from Escherichia coli

Name: Thioredoxin from <I>Escherichia coli</I>
Brand: SIGMA

recombinant, expressed in E. coli, essentially salt-free, lyophilized powder, ≥3 units/mg protein

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About This Item

CAS Number:

52500-60-4

UNSPSC Code:

12352200

NACRES:

NA.32

MDL number:

MFCD00132423

Form:

essentially salt-free, lyophilized powder

Assay:

≥90% (SDS-PAGE)

Biological source:

Escherichia coli

Recombinant:

expressed in E. coli

Mol wt:

11.7 kDa

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Quality Level

200

biological source

Escherichia coli

recombinant

expressed in E. coli

assay

≥90% (SDS-PAGE)

form

essentially salt-free, lyophilized powder

specific activity

≥3 units/mg protein

mol wt

11.7 kDa

technique(s)

activity assay: suitable

solubility

water: 1.90-2.10 mg/mL, clear, colorless

Protein ID accession no.

M26133

UniProt accession no.

P0AA25

application(s)

cell analysis

storage temp.

−20°C

Gene Information

Escherichia coli K12 ... trxA(948289)

General description

Research area: Cell Signaling

Thioredoxin from Escherichia coli is a monomeric, globular protein. It has a central β-sheet surrounded by α-helices.

Application

Thioredoxin from Escherichia coli has been used in thioredoxin reductase assay, peroxidase-coupled thioredoxin system assay and peroxiredoxin assay. It has also been used to study its effects on photoreceptor death in a mouse model of retinitis pigmentosa (RP) and its potential to modify retinal oxidative stress, glial changes, and vascular changes during the progression of the disease.

Biochem/physiol Actions

Mediates the reduction of disulfide bonds in proteins.

Thioredoxin from Escherichia coli catalyzes REDOX reactions and has a role in various biological processes. It modulates the structure and activity of many proteins. The enzyme thioredoxin reductase catalyzes the reduction of thioredoxin. Thioredoxin also has a role in oxidative stress response.Thioredoxin, known as both adult T cell leukemia-derived factor (ADF) and a cellular redox enzyme, functions as a cytokine or chemokine, facilitating cell-to-cell communication. Additionally, it plays a role in regulating cell growth and apoptosis.

Other Notes

GenBank^® Accession Number M26133.

One unit will cause a ΔA₆₅₀ of 1.0 in 1 min at 25 °C in the insulin reduction assay.

Legal Information

GenBank is a registered trademark of United States Department of Health and Human Services

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

T0910-BULK: + T0910-1MG-PW: + T0910-1MG: + T0910-VAR:

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Thioredoxin from Escherichia coli as a Role Model of Molecular Recognition, Folding, Dynamics and Function.

Vazquez DS

Protein and peptide letters, 22(9), 801-815 (2015)

Proteomic analysis of thioredoxin-targeted proteins in Escherichia coli.

Kumar JK

Proceedings of the National Academy of Sciences of the USA, 101(11), 3759-3764 (2004)

Mechanism and structure of thioredoxin reductase from Escherichia coli.

Williams CH Jr

Faseb Journal, 9(13), 1267-1276 (1995)

Ohr (organic hydroperoxide resistance protein) possesses a previously undescribed activity, lipoyl-dependent peroxidase.

Cussiol JR

The Journal of Biological Chemistry, 285(29), 21943-21950 (2010)

Intracellular Pathways of Holothuroid Oocyte Maturation Induced by the Thioredoxin Trx-REES.

Jérôme Delroisse et al.

Antioxidants (Basel, Switzerland), 10(8) (2021-08-28)

In holothuroids, oocyte maturation is stopped in ovaries at the prophase I stage of meiosis. In natural conditions, the blockage is removed during the spawning by an unknown mechanism. When oocytes are isolated by dissection, the meiotic release can be

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