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T2507

Triosephosphate Isomerase from baker′s yeast (S. cerevisiae)

Name: Triosephosphate Isomerase from baker′s yeast (<I>S. cerevisiae</I>)
Brand: SIGMA

Type I, ammonium sulfate suspension, ~10,000 units/mg protein

Synonym(s):

D-Glyceraldehyde-3-phosphate ketol-isomerase, TPI

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About This Item

CAS Number:

9023-78-3

UNSPSC Code:

12352204

NACRES:

NA.54

EC Number:

232-774-2

MDL number:

MFCD00082080

EC Number:

5.3.1.1 (BRENDA, IUBMB)

Specific activity:

~10,000 units/mg protein

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Properties

type

Type I

Quality Level

200

form

ammonium sulfate suspension

specific activity

~10,000 units/mg protein

mol wt

53 kDa by equilibrium centrifugation

foreign activity

pyruvate kinase, lactic dehydrogenase, 3-phosphoglyceric phosphokinase, phosphoglucose isomerase, α-glycerophosphate dehydrogenase, aldolase, and glyceraldehyde-3-phosphate dehydrogenase <0.01%

storage temp.

2-8°C

Description

Application

Triosephosphate isomerase has been used in a study to assess differential expression of fourteen proteins of uveal melanoma. Triosephosphate isomerase has also been used in a study to investigate the use of sigmoid pH gradients in free-flow isoelectric focusing of human endothelial cell proteins.

Physical form

Crystalline suspension in 2.7 M (NH₄)₂SO₄, 0.5 mM EDTA, pH 6.5

Analysis Note

Protein determined by biuret.

Other Notes

One unit will convert 1.0 μmole D-glyceraldehyde 3-phosphate to dihydroxyacetone phosphate per min at pH 7.6 at 25 °C.

Since the assay of this enzyme seems to be sensitive to minor variables, packages are dispensed per mg protein rather than in units.

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

T2507-2.5MG: + T2507-BULK-N: + T2507-5MG: + T2507-VAR-N: + T2507-10MG: + T2507-BULK: + T2507-.5MG: + T2507-VAR: + T2507-25MG:

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Certificates of Analysis (COA)

Lot/Batch Number

060H8110

036H8025

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Articles

硫酸アンモニウム懸濁液として提供される酵素の使用方法

How to Work with Enzymes Supplied as Ammonium Sulfate Suspensions

Instructions for working with enzymes supplied as ammonium sulfate suspensions