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Merck

T6557

Anti-γ-Tubulin Antibody

mouse monoclonal, GTU-88

Synonym(s):

Anti-γ-Tubulin antibody, Mouse monoclonal

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41
MDL number:
MFCD00677366
Conjugate:
unconjugated
Clone:
GTU-88, monoclonal
Application:
ELISA (i), ICC, WB
Citations:
1134

Key Documents

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Product Name

Anti-γ-Tubulin antibody, Mouse monoclonal, clone GTU-88, ascites fluid

biological source

mouse

conjugate

unconjugated

antibody form

ascites fluid

antibody product type

primary antibodies

clone

GTU-88, monoclonal

mol wt

antigen 48 kDa

contains

15 mM sodium azide

species reactivity

rat, hamster, chicken, human, bovine, canine, Xenopus, mouse

technique(s)

immunocytochemistry: 1:5,000-1:10,000 using HeLa cells, indirect ELISA: suitable, western blot: 1:10,000 using cultured chicken fibroblast extract

isotype

IgG1

UniProt accession no.

P23258

application(s)

research pathology

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Quality Level

200

Gene Information

human ... TUBG1(7283)
mouse ... Tubg1(103733)
rat ... Tubg1(252921)

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General description

γ-Tubulin (48kDa) is a ubiquitous and highly conserved protein within the microtubule organizing centers (MTOCs) in eukaryotic cells. γ-Tubulin is mapped to human chromosome 17q21.2 and codes for a member of the tubulin family. Human TUBG1 transcript is widely expressed in preimplantation embryos and brain.
Monoclonal Anti-γ-Tubulin (mouse IgG1 isotype) is derived from the GTU-88 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse.

Immunogen

synthetic γ-tubulin peptide, conjugated to KLH

Application

Monoclonal Anti-γ-Tubulin antibody has been used in western blotting, indirect immunofluorescence (IF) and immunofluorescence staining.
Monoclonal Anti-γ-Tubulin is also suitable for use in immunochemical applications such as immunoblotting, immunocytochemical staining of cultured cells and in ELISA.
Monoclonal Anti-gamma-Tubulin is suitable for use in immunochemical applications such as immunoblotting, immunocytochemical staining of cultured cells, and in ELISA.

Biochem/physiol Actions

γ-Tubulin nucleates microtubule assembly throughout the mammalian cell cycle in vivo. γ-Tubulin binds microtubule minus ends and is responsible for mediating the link between microtubules and the centrosome. It functions as the microtubule nucleator at the MTOC. It binds to the β-tubulin half of the tubulin molecule, thus establishing the polarity of a microtubule, leaving the α-tubulin half exposed at the plus end. γ-Tubulin abundance is less than 1% of the level of either α- or β-tubulin. Overexpression of γ-tubulin is observed in lung cancer. The expression levels of γ-tubulin can be considered as an important prognostic indicator for patients with astrocytomas.
Monoclonal Anti-γ-Tubulin recognizes an epitope located in the N-terminal amino acids of γ-tubulin (48 kDa). Cross reactivity has been observed with human, bovine, dog, hamster, rat, mouse, chicken, and Xenopus γ-tubulin.
The antibody recognizes an epitope located within the N-terminal region of γ-tubulin.

Physical form

The product is provided as ascites fluid with 15 mM sodium azide as a preservative.

Preparation Note

For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in "frostfree" freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

10 - Combustible liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

T6557-.2ML: + T6557-VAR: + T6557-BULK: + T6557-100UL: + T6557-.5ML:

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Certificates of Analysis (COA)

Lot/Batch Number
049M4786V
22181236
088M4786V
028M4840V
067M4857V

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Human mineralocorticoid receptor (MR) gene haplotypes modulate MR expression and transactivation: implication for the stress response.
Van Leeuwen N, et al.
Psychoneuroendocrinology, 36(5), 699-709 (2011)
Downregulation of connexin43 by microRNA-130a in cardiomyocytes results in cardiac arrhythmias
Osbourne A, et al.
Journal of Molecular and Cellular Cardiology, 74, 53-63 (2014)
Gamma-tubulin is a minus end-specific microtubule binding protein.
Li Q and Joshi HC
The Journal of Cell Biology, 131(1), 207-214 (1995)
Overexpression of γ-tubulin in non-small cell lung cancer.
Maounis NF, et al.
Histology and Histopathology, 27(7), 1183-1183 (2012)
Interaction of amyotrophic lateral sclerosis (ALS)-related mutant copper-zinc superoxide dismutase with the dynein-dynactin complex contributes to inclusion formation.
Strom AL, et al.
The Journal of Biological Chemistry, 283(33), 22795-22805 (2008)
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Articles

Antibodies to Tubulins, including Centrosome-Associated ε-Tubulin

Microtubules of the eukaryotic cytoskeleton are composed of a heterodimer of α- and β-tubulin. In addition to α-and β-tubulin, several other tubulins have been identified, bringing the number of distinct tubulin classes to seven.

εチューブリンを含むチューブリンに対する抗体

αチューブリンとβチューブリンのヘテロダイマーで構成される真核細胞の細胞骨格の微小管には、多様な機能を発揮する複数の明確なチューブリンクラスがあります。

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