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Merck

53829-U

Ascentis® Express C18 (2.7 μm) HPLC Column

L × I.D. 15 cm × 4.6 mm, pk of 1 ea

Synonym(s):

Core-shell (SPP) Fused Core C18 HPLC column

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About This Item

UNSPSC Code:
41115700
NACRES:
SB.52
eCl@ss:
32110501
L × i.d.:
15 cm × 4.6 mm
Particle size:
2.7 μm
Matrix active group:
C18 (octadecyl) phase
Pore size:
90 Å
Matrix:
Fused-Core particle platform, superficially porous particle
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material

stainless steel column

Quality Level

agency

suitable for USP L1

product line

Ascentis®

feature

endcapped

packaging

pk of 1 ea

manufacturer/tradename

Ascentis®

parameter

60 °C temp. range, 600 bar max. pressure (9000 psi)

technique(s)

HPLC: suitable, LC/MS: suitable, UHPLC-MS: suitable, UHPLC: suitable

L × I.D.

15 cm × 4.6 mm

surface area

135 m2/g

impurities

<5 ppm metals

matrix

Fused-Core particle platform, superficially porous particle

matrix active group

C18 (octadecyl) phase

particle size

2.7 μm

pore size

90 Å

operating pH

2-9

application(s)

food and beverages

separation technique

reversed phase

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General description

Ascentis Express HPLC columns, through the use of Fused-Core® particle technology, can provide you with both the high speed and high efficiencies of sub-2 μm particles while maintaining lower backpressures. The combination of high efficiency and low backpressure benefits UPLC® (or other ultra high pressure system) users, as well as conventional HPLC users.
Visit the Ascentis Express home page for more information on this new column technology.

Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany
Fused-Core is a registered trademark of Advanced Materials Technology, Inc.
UPLC is a registered trademark of Waters

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Silvia Jakabová et al.
Journal of chromatography. A, 1232, 295-301 (2012-03-07)
Hyoscyamine (atropine) and scopolamine are the predominant tropane alkaloids in the Datura genus, occurring in all plant organs. The assessment of the alkaloid content of various plant parts is essential from the viewpoint of medical use, but also as a
Judy Stone
Methods in molecular biology (Clifton, N.J.), 1378, 301-320 (2015-11-26)
Serum from bar-coded tubes, and then internal standard, are pipetted to 96-well plates with an 8-channel automated liquid handler (ALH). The first precipitation reagent (methanol:ZnSO4) is added and mixed with the 8-channel ALH. A second protein precipitating agent, 1 %
Babu Rao Chandu et al.
SpringerPlus, 2(1), 194-194 (2013-06-07)
A bioequivalence study was proved of generic Febuxostat 80 mg tablets (T) in healthy volunteers.For this purpose, Authors developed a simple, sensitive, selective, rapid, rugged and reproducible liquid chromatography-tandem mass spectrometry method for the quantification of Febuxostat (FB) in human
Petra Šilarová et al.
Food chemistry, 237, 471-480 (2017-08-03)
The degradation of catechins and other phenolics in green tea infusions were monitored using fast HPLC/MS separation. The final separation was performed within 2.5min using Ascentis Express C18 column (50mm×2.1mm i.d.) packed with 2μm porous shell particles. Degradation was studied
Pankaj Partani et al.
Journal of chromatographic science, 54(8), 1385-1396 (2016-05-27)
A liquid chromatography-tandem mass spectrometry method was developed and validated for the simultaneous determination of simvastatin (SV) and simvastatin acid (SVA) in human plasma. To improve assay sensitivity and achieve simultaneous analysis, SVA monitored in (-)ESI (electrospray ionization) mode within

Articles

Optimize HPLC method for ginsenoside separation using a mixture, applying it to American Ginseng root, with conditions and chromatograms shown.

Derivatization of Aliphatic Amines - Results from the Supelco Ascentis Express column show higher peak resolution, and improved peak shapes than typical chromatograms.

USP methods continuously revised for modernization and improved results in drug testing.

混合物を用いたジンセノサイド分離のためにHPLC法を最適化し、その方法をアメリカ人参根に応用します。実験条件とクロマトグラムを示します。

Protocols

Investigate bile acid roles in gut hormone profiles and glycemic control, vital for clinical labs exploring potential mechanisms.

Lopinavir assay setup saves time and improves efficiency with shorter columns, meeting European pharmacopoeia guidelines.

-methylcarbamate 10 μg/mL; Diuron; Propham; Siduron; Methiocarb, analytical standard; Linuron 10 μg/mL; Swep 10 μg/mL; Chlorpropham 10 μg/mL; Barban; Neburon

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Ibuprofen and impurity separation using Ascentis Express 90Å C18 column with excellent peak shape and low quantification limits.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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