04-1624
Anti-Centrin Antibody, clone 20H5
clone 20H5, from mouse
別名:
Caltractin isoform 2, EF-hand protein, calcium binding protein, centrin 1, centrin, EF-hand protein, 1
製品名
Anti-Centrin Antibody, clone 20H5, clone 20H5, from mouse
biological source
mouse
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
20H5, monoclonal
species reactivity
rat, human, mouse
technique(s)
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
isotype
IgG2aκ
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Quality Level
Gene Information
human ... CETN1(1068)
Analysis Note
Control
HeLa cell lysate
HeLa cell lysate
Evaluated by Western Blot in HeLa cell lysate.
Western Blot Analysis: 1 µg/ml of this antibody detected Centrin on 10 µg of HeLa cell lysate.
Western Blot Analysis: 1 µg/ml of this antibody detected Centrin on 10 µg of HeLa cell lysate.
Application
Anti-Centrin Antibody, clone 20H5 is a Mouse Monoclonal Antibody for detection of Centrin also known as Caltractin isoform 2, EF-hand protein & has been validated in WB, IP, ICC.
Immunocytochemistry Analysis: Manandhar, G., et al. (1999). Cell Motil Cytoskeleton. 43(2):137-144.
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Cell Cycle, DNA Replication & Repair
Cell Cycle, DNA Replication & Repair
Biochem/physiol Actions
This antibody recognizes the C-terminus of Centrin.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description
Centrin is a key regulator of centriole duplication. This protein is localized to the centrosome of interphase cells. During mitosis, centrin redistributes to the spindle poles, consistent with dynamic changes of the centrosome during the cell cycle. Current research suggests centrin exerts spatial and temporal control over centriole duplication through its role as a component of centriole precursor structures and through periodic cell-cycle specific changes in its abundance.
~ 20 kDa
Immunogen
Epitope: C-terminus
Recombinant protein corresponding to the C-terminus of Chlamydomonas Centrin.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Physical form
Format: Purified
Protein G Purified
Purified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
適切な製品が見つかりませんか。
製品選択ツール.をお試しください
保管分類
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
Tamara A Potapova et al.
Molecular biology of the cell, 27(20), 3065-3084 (2016-08-26)
Tetraploidization, or genome doubling, is a prominent event in tumorigenesis, primarily because cell division in polyploid cells is error-prone and produces aneuploid cells. This study investigates changes in gene expression evoked in acute and adapted tetraploid cells and their effect
G Manandhar et al.
Cell motility and the cytoskeleton, 43(2), 137-144 (1999-06-24)
Centrosome reduction during mouse spermiogenesis has been studied by immunofluorescent microscopy using anticentrin antibody (20H5) and TEM. Centrin is detected as two spots in round spermatids, corresponding to a pair of centrioles. In elongating spermatids, centrin spots colocalize with the
Stress-induced localization of HSPA6 (HSP70B') and HSPA1A (HSP70-1) proteins to centrioles in human neuronal cells.
Khalouei, S; Chow, AM; Brown, IR
Cell Stress & Chaperones null
Mi Young Seo et al.
PloS one, 10(9), e0138905-e0138905 (2015-09-26)
A procentriole is assembled next to the mother centriole during S phase and remains associated until M phase. After functioning as a spindle pole during mitosis, the mother centriole and procentriole are separated at the end of mitosis. A close
Rachael A McCloy et al.
Cell cycle (Georgetown, Tex.), 13(9), 1400-1412 (2014-03-15)
Entry and progression through mitosis has traditionally been linked directly to the activity of cyclin-dependent kinase 1 (Cdk1). In this study we utilized low doses of the Cdk1-specific inhibitor, RO3306 from early G 2 phase onwards. Addition of low doses
グローバルトレードアイテム番号
| カタログ番号 | GTIN |
|---|---|
| 04-1624 | 04053252672200 |
ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.
製品に関するお問い合わせはこちら（テクニカルサービス）