Anti-Ubiquitin Antibody, Lys48-Specific, clone Apu2, rabbit monoclonal

Western Blot Analysis:
A 1:1000-1:5000 dilution of this lot detected Lys-48 ubiquitin chains by Western blotting.

Control
Lys48 polyubiquitin chains.

Immunohistochemistry:
Ubiquitin (Lys 48-specific) staining of rat brain sections (left figure) or human breast carcinoma (right figure). Staining pattern detects axons of neurons in brain tissue but not surrounding glial cells, and breast tumor cell cytoplasm is strongly detected without nuclear involvement.

Immunocytochemistry:
Confocal IF analysis of NIH/3T3, HeLa, and A431 cells using anti-Ubiquitin (Lys48 specific) (Red). Actin filaments were labeled with AlexaFluor 488-Phalloidin (Green). Nuclear material is stained with DAPI (Blue).

Flow cytometry:
Flow cytometry analysis of Jurkat cells using Anti-ubiquitin Lys48-specific (#05-1307) (blue) and rabbit IgG control (red).

Immunoprecipitation:
Rabbit monoclonal anti-ubiquitin (Lys-48-specific), clone Apu2, precipitated ubiquitin-containing proteins from HeLa cell lysates.

Inhibition Assay:
Preincubation of anti-ubiquitin, Lys48-specific, with K48-multiubiquitin chains inhibited binding to HeLa cell lysate in Western blots.

Anti-Ubiquitin Antibody, Lys48-Specific, clone Apu2 is a high quality Rabbit Monoclonal Antibody for the detection of Ubiquitin & has been validated in FC, ICC, IP, IHC, WB, PIA.

Research Category
Signaling

Research Sub Category
Ubiquitin & Ubiquitin Metabolism

Based on sequence homology, broad species cross-reactivity is expected.

Recognizes ubiquitin linked at Lys48. Does not recognize ubiquitin linked at Lys63 or free ubiquitin.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

DI-UBIQUITIN PROTEIN AVAILABLE!
Di-Ubiquitin (Lys48-linked) Recombinant Protein (17-10408)

Polyubiquitin chains linked through the Lys48 residue of ubiquitin are most commonly associated with proteins targeted for proteosomal degradation.

In contrast, polyubiquitin chains linked through the Lys63 residue of ubiquitin (Ub) are associated with nonproteolytic functions such as signal transduction. Lys63-linked polyUb chains connect components of NFκB signaling in a highly regulated manner, and genetic evidence indicates the involvement of Lys63-linked Ub chains in stress response and DNA repair. Research in yeast has suggested Lys63-linked Ub chains stimulate endocytosis.

varies

Epitope: Lys48 ubiquitination site

Immunogen used was human di-ubiquitin linked at Lys48.

Format: Purified

Protein A purified

Protein A purified recombinant purified recombinant rabbit monoclonal antibody that consists of a humanized fAb fragment fused to a rabbit Fc tail produced in a CHO recombinant antibody system. Supplied in 0.1M Tris-Glycine (pH7.4) 150 mM NaCl, 0.05% sodium azide

Stable for 1 year at 2-8°C from date of receipt.
For maximum recovery of product, centrifuge the vial prior to removing the cap.