Anti-Ubiquitin, Lys48-Specific, clone Apu2, Alexa Fluor^™ 488 conjugate

Polyubiquitin-B (UniProt P0CG47) is encoded by the UBB gene (Gene ID 7314) in human. Ubiqutin (Ub) is initially produced as a 229-a.a. precursor protein whose C-terminus Cys229 is post-translationally removed, followed by cleavages between a.a. 76 and 77, as well as between a.a. 152 and 153 to yield three idential 76-amino acid Ub molecules. Ub is involved in one of the most common post-translational modifications (PTMs) of cellular proteins, where Ub is linked covalently via its carboxyl terminus to usually Lys residues in target proteins. A given target Lys residue can be linked to one single Ub molecule (monoubiquitylated) or to a chain of Ub molecules (polyubiquitylated). In a polyubiquitin chain, ubiquitin molecules can be linked through one of the seven ubiquitin Lys residues (K6, K11, K27, K29, K33, K48, and K63) or through the Ub N-terminus Met1 residue (which generates linear chains). An Ub chain with a single linkage type is referred to as homotypic, whereas an heterotypic chain contains mixed linkages within the same chain, which can lead to branched/forked (as a result of one Ub linked to two or more Ubs) or non-branched Ub chains. Lys48-linked chains mark proteins for proteasomal degradation, while Lys63-linked chains regulate the activity/function of cellular proteins.

~17 kDa observed

Epitope: Lys48 ubiquitination site

Immunogen used was human di-ubiquitin linked at Lys48.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Ubiquitin in A431 and NIH/3T3 cells.
The unconjugated antibody (Cat. No. 05-1307) is shown to be suitable also for cell funtion assay, flow cytometry, immunocytochemistry, immunohistochemistry, immunoprecipitation, Western blotting applications.

Research Category
Signaling

Research Sub Category
Developmental Signaling

This Anti-Ubiquitin, Lys48-Specific, clone Apu2, Alexa Fluor^™ 488 conjugate is validated for use in Immunocytochemistry for the detection of Ubiquitin.

Based on sequence homology, broad species cross-reactivity is expected.

Recognizes ubiquitin linked at Lys48. Does not recognize ubiquitin linked at Lys63 or free ubiquitin.

Protein A purified

Purified rabbit antibody conjugate in PBS with 15 mg/ml BSA and 0.1% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Evaluated by Immunocytochemistry in HeLa cells.

Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected Ubiquitin in HeLa cells.

Concentration: Please refer to lot specific datasheet.

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