Anti-RAD21 Antibody

100-120kDa

Aka Scc1, part of the cohesin complex, Rad21 is a nuclear protein that associates with mitotic chromatin and is essential for the association of kinetochores with microtubules. Rad21/Scc1 s is cleaved by caspases, promoting apoptosis.

The protein encoded by the Rad21 gene is highly similar to the gene product of Schizosaccharomyces pombe rad21, a gene involved in the repair of DNA double-strand breaks, as well as in chromatid cohesion during mitosis. This protein is a nuclear phospho-protein, which becomes hyperphosphorylated in cell cycle M phase.
RAD21 also plays a role in apoptosis, via its cleavage by caspase 3 or caspase 7 during early steps of apoptosis: the C-terminal 64 kDa cleavage product may act as a nuclear signal to initiate cytoplasmic events involved in the apoptotic pathway. Recent work has shown that the RAD21 has a role in the invasion and metastasis of cancer cells

GST fusion protein corresponding to residues 240-631 of human Rad21

Detect RAD21 with Anti-RAD21 Antibody (Mouse Monoclonal Antibody), that has been shown to work in IP, WB, ICC.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Cell Cycle, DNA Replication & Repair

Rad21

Format: Purified

Protein G Purified

Prtotein G purified antibody in 0.1M Tris-Glycine, 0.15M NaCl, 0.05% Sodium Azide, pH 7.4

Stable for 1 year at 2-8°C from date of receipt.

Control
Positive Antigen Control: Catalog #12-309, Hela cell nuclear extract. Add an equal volume of Laemmli reducing sample buffer to 10 μL of extract and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced extract per lane for minigels.

Routinely evaluated by immunoblot with HeLa nuclear extracts

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