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Merck

07-850

Anti-phospho-LIMK 1/2 (Tyr507/Thr508) Antibody

Upstate®, from rabbit

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この商品について

UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702

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製品名

Anti-phospho-LIMK 1/2 (Tyr507/Thr508) Antibody, Upstate®, from rabbit

biological source

rabbit

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

human

manufacturer/tradename

Upstate®

technique(s)

western blot: suitable

isotype

IgG

NCBI accession no.

NM_002314.2

UniProt accession no.

P53667
P53671

shipped in

dry ice

target post-translational modification

phosphorylation (pTyr507/pThr508)

Quality Level

100

Gene Information

human ... LIMK1(3984)

Analysis Note

Routinely evaluated by immunoblot.

Application

Detect phospho-LIMK 1/2 (Tyr507/Thr508) using this anti-phospho-LIMK 1/2 (Tyr507/Thr508) Antibody validated for use in WB.
Immunoblot Analysis: A 1:1000 dilution of this lot detected phosphorylated LIMK 1/2 (Tyr507/Thr508) in RIPA lysates of SDF-1α treated Jurkat cells.
Research Category
Signaling
Research Sub Category
Cytoskeletal Signaling

Biochem/physiol Actions

Predicted to cross-react with Mouse, Rat, and Frog (100% homologous) based on sequence homology.
Recognizes phosphorylated LIMK1/2 (Tyr507/Thr508)

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

72 kDa (LIMK1) and 65 kDa (LIMK2)

Immunogen

Peptide corresponding to amino acid region encompassing the human, mouse, rat and chicken phospho-LIMK1/2 (Tyr507/Thr508)

Physical form

100 μL of affinity purified rabbit polyclonal IgG in 50% storage buffer (PBS (without Mg2+ and Ca2+), pH 7.3 containing 1.0 mg/mL BSA (IgG, protease free) and 0.05% sodium azide) and 50% glycerol.
ImmunoAffinity Purified

Preparation Note

Stable for 2 years at -20°C from date of shipment. Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol-containing solutions to become frozen during storage.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

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保管分類

12 - Non Combustible Liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

試験成績書（COA）

製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。

以前この製品を購入いただいたことがある場合

文書ライブラリで、最近購入した製品の文書を検索できます。

文書ライブラリにアクセスする

Down-regulation of PKCzeta expression inhibits chemotaxis signal transduction in human lung cancer cells.
Ying Liu,Bing Wang,Jingna Wang,Wuzhou Wan,Ronghua Sun,Yuliang Zhao,Ning Zhang
Lung Cancer null
Juliana Soosairajah et al.
The EMBO journal, 24(3), 473-486 (2005-01-22)
Slingshot (SSH) phosphatases and LIM kinases (LIMK) regulate actin dynamics via a reversible phosphorylation (inactivation) of serine 3 in actin-depolymerizing factor (ADF) and cofilin. Here we demonstrate that a multi-protein complex consisting of SSH-1L, LIMK1, actin, and the scaffolding protein
D C Edwards et al.
Nature cell biology, 1(5), 253-259 (1999-11-13)
Extracellular signals regulate actin dynamics through small GTPases of the Rho/Rac/Cdc42 (p21) family. Here we show that p21-activated kinase (Pak1) phosphorylates LIM-kinase at threonine residue 508 within LIM-kinase's activation loop, and increases LIM-kinase-mediated phosphorylation of the actin-regulatory protein cofilin tenfold
K Ohashi et al.
The Journal of biological chemistry, 275(5), 3577-3582 (2000-02-01)
LIM-kinase 1 (LIMK1) phosphorylates cofilin, an actin-depolymerizing factor, and regulates actin cytoskeletal reorganization. LIMK1 is activated by the small GTPase Rho and its downstream protein kinase ROCK. We now report the site of phosphorylation of LIMK1 by ROCK. In vitro

グローバルトレードアイテム番号

カタログ番号GTIN
07-85004053252581434

ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.

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