description
Merck USA index - 14, 8468
assay
≥98% (agarose gel electrophoresis)
form
solid
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
impurities
≤3.0 ash
≤5.0% moisture
color
off-white
solubility
0.15 M sodium chloride: 10 mg/mL
cation traces
heavy metals: ≤10 ppm
shipped in
ambient
storage temp.
2-8°C
Application
Albumin, Bovine Serum, Fraction V, RIA and ELISA Grade has been used:
- to block tissue sections of placenta in immunohistochemical (IHC) analysis
- as a component of Krebs buffer for in vitro glucose-stimulated insulin secretion (GSIS) experiments and drug/palmitate preparation
- as a vehicle control to incubate whole mouse blood to study plate aggregation in vivo
Biochem/physiol Actions
Bovine Serum Albumin (BSA), an important component in the cell culture media can transport fatty acids, hormones, and drugs. It is generally used as a blocking agent in enzyme-linked immunosorbent assay (ELISA).
Disclaimer
Toxicity: Standard Handling (A)
General description
Albumin, Bovine Serum, Fraction V, RIA and ELISA Grade is a purified plasma protein that is useful as a stabilizing agent and as a carrier protein. It is prepared under non-denaturing conditions by modification of the Cohn procedure and caprylic acid heat-shock treatment.
Bovine serum albumin (BSA) is an α-helical, non-glycosylated globular protein with 17-disulfide bonds.
Preparation Note
Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
保管分類
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
試験成績書(COA)
製品のロット番号・バッチ番号を入力して、試験成績書(COA) を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
Olga Troitskaya et al.
International journal of molecular sciences, 22(23) (2021-12-11)
Multicellular spheroids with 3D cell-cell interactions are a useful model to simulate the growth conditions of cancer. There is evidence that in tumor spheroids, the expression of various essential molecules is changed compared to the adherent form of cell cultures.
Amber Lockridge et al.
Cell reports, 31(5), 107609-107609 (2020-05-07)
During early obesity, pancreatic β cells compensate for increased metabolic demand through a transient phase of insulin hypersecretion that stabilizes blood glucose and forestalls diabetic progression. We find evidence that β cell O-GlcNAcylation, a nutrient-responsive post-translational protein modification regulated by
Tamara Topală et al.
Clujul medical (1957), 87(4), 215-219 (2014-01-01)
The continuous search for new molecules with therapeutic abilities has led to the synthesis and characterization of a large number of metal complexes, proven to exhibit potential as pharmacological agents through their antibacterial, antiviral, antifungal and antineoplastic properties. As serum
Yuhong Xiao et al.
Journal of immunological methods, 384(1-2), 148-151 (2012-06-27)
The enzyme-linked immunosorbent assay (ELISA) is an extremely common and powerful laboratory technique for detecting proteins by antibodies. Researchers frequently use bovine serum albumin (BSA) as a blocking agent to prevent non-specific binding of antigens and antibodies to the microtiter
グローバルトレードアイテム番号
| カタログ番号 | GTIN |
|---|---|
| 126593-10GMCN | 04055977222593 |
| 126593-100GMCN | 07790788048082 |
| 126593-25GMCN | 04055977222616 |
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