14-420
MEK1 Protein, inactive, 50 g
Unactive, N-terminal GST & C-terminal His6-tagged, recombinant full-length human MEK1, for use in Kinase Assays.
別名:
MEK1 Protein
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この商品について
UNSPSC Code:
12352202
NACRES:
NA.41
eCl@ss:
32160405
Biological source:
human
Recombinant:
expressed in E. coli
Mol wt:
Mw 71 kDa
biological source
human
recombinant
expressed in E. coli
mol wt
Mw 71 kDa
manufacturer/tradename
Upstate®
technique(s)
activity assay: suitable (kinase)
NCBI accession no.
UniProt accession no.
shipped in
dry ice
Quality Level
Gene Information
human ... MAP2K1(5604)
Analysis Note
routinely evaluated by phosphorylation of MBP (Catalog # 13-104) substrate in vitro
Application
Mitogen-Activated Protein Kinase Kinase 1 has been used in in vitro kinase assays:
- to elucidate the underlying mechanisms involved in the response to Rapidly Accelerated Fibrosarcoma (RAF) inhibitors and the interactions with downstream pathways.
- to investigate the role of Mitogen-Activated Protein Kinase Kinase Kinase 2 (MEKK2) as a MAP3K in the Extracellular Signal-Regulated Kinase (ERK) pathway in osteoblasts and to assess its potential as a therapeutic target for treating Neurofibromatosis Type 1 (NF1).
- to assess the effectiveness of the combined targeted therapy with Dabrafenib and Trametinib in treating non-small cell lung cancers (NSCLCs).
Biochem/physiol Actions
Protein Target: MEK1
Target Sub-Family: STE Mitogen-Activated Protein Kinase Kinase 1 (MEK1) is involved in the RAS-RAF-MEK-ERK signaling pathway. This pathway plays a crucial role in various cellular processes, including apoptosis, cell cycle progression, migration, differentiation, metabolism, and proliferation. One of its important functions is the phosphorylation of specific sites in extracellular signal-regulated kinases (ERKs) Mitogen-Activated Protein Kinase /Extracellular Signal-Regulated Kinase (MAPK3/ERK1 and MAPK1/ERK2). MEK activation is closely associated with stimulating cell growth and transformation processes.
Target Sub-Family: STE Mitogen-Activated Protein Kinase Kinase 1 (MEK1) is involved in the RAS-RAF-MEK-ERK signaling pathway. This pathway plays a crucial role in various cellular processes, including apoptosis, cell cycle progression, migration, differentiation, metabolism, and proliferation. One of its important functions is the phosphorylation of specific sites in extracellular signal-regulated kinases (ERKs) Mitogen-Activated Protein Kinase /Extracellular Signal-Regulated Kinase (MAPK3/ERK1 and MAPK1/ERK2). MEK activation is closely associated with stimulating cell growth and transformation processes.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description
Research area: CELL SIGNALING
N-terminal GST, and C-terminal His6-tagged, recombinant full-length human MEK1
Product Source: expressed in E.coli
MEK1, also known as Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1), is a protein kinase located on chromosome 15q22.1-q22.33, consisting of 12 exons and 393 amino acids. The protein is made up of two lobes containing several conserved α-helices and β-strands.
N-terminal GST, and C-terminal His6-tagged, recombinant full-length human MEK1
Product Source: expressed in E.coli
MEK1, also known as Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1), is a protein kinase located on chromosome 15q22.1-q22.33, consisting of 12 exons and 393 amino acids. The protein is made up of two lobes containing several conserved α-helices and β-strands.
Other Notes
For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
Physical form
glutathione-agarose followed by Ni2+/NTA-agarose
Preparation Note
6 months at -70°C
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
signalword
Warning
hcodes
Hazard Classifications
Skin Sens. 1
保管分類
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Deleterious substance
pdsc
14-420:
jan
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
Ingo V Hartung et al.
ChemMedChem, 10(12), 2004-2013 (2015-11-07)
Having recently identified a so-far unexplored area adjacent to the known binding site of allosteric mitogen-activated protein kinase kinase (MEK) inhibitors, we now report an extension of these studies by combining our new side chains with different MEK inhibitor cores
David S Nelson et al.
Genes, chromosomes & cancer, 54(6), 361-368 (2015-04-23)
Langerhans cell histiocytosis (LCH) is now understood to be a neoplastic disease in which over 50% of cases have somatic activating mutations of BRAF. However, the extracellular signal-related (ERK) pathway is activated in all cases including those with wild type
P Zhang et al.
The Journal of biological chemistry, 275(29), 22479-22486 (2000-05-10)
Activation of ERK-1 and -2 by H(2)O(2) in a variety of cell types requires epidermal growth factor receptor (EGFR) phosphorylation. In this study, we investigated the activation of ERK by ONOO(-) in cultured rat lung myofibroblasts. Western blot analysis using
O Zugasti et al.
Molecular and cellular biology, 21(19), 6706-6717 (2001-09-05)
Signals from the extracellular matrix are essential for the survival of many cell types. Dominant-negative mutants of two members of Rho family GTPases, Rac1 and Cdc42, mimic the loss of anchorage in primary mouse fibroblasts and are potent inducers of
M Dolores García-Fernández et al.
Cell reports methods, 1(8), None-None (2022-01-04)
Ligand-gated ion channels (LGICs) are natural biosensors generating electrical signals in response to the binding of specific ligands. Creating de novo LGICs for biosensing applications is technically challenging. We have previously designed modified LGICs by linking G protein-coupled receptors (GPCRs)
グローバルトレードアイテム番号
| カタログ番号 | GTIN |
|---|---|
| 14-420 | 04053252365263 |
ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.
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