コンテンツへスキップ
Merck

17-601

ChIPAb+ Sp1 - ChIP Validated Antibody and Primer Set

from rabbit

別名:

Transcription factor Sp1, TSFP1

ログインで組織・契約価格をご覧ください。

サイズを選択してください

この商品について

UNSPSCコード:
12352203
eCl@ss:
32160702
NACRES:
NA.41

主要文書

すべての文書を表示
テクニカルサービス
お困りのことがあれば、経験豊富なテクニカルサービスチームがお客様をサポートします。
お手伝いします
テクニカルサービス
お困りのことがあれば、経験豊富なテクニカルサービスチームがお客様をサポートします。
お手伝いします

由来生物

rabbit

品質水準

100

クローン

polyclonal

交差性

rat, human, mouse

メーカー／製品名

ChIPAb+
Upstate®

テクニック

ChIP: suitable
western blot: suitable

NCBIアクセッション番号

NM_138473.2

UniProtアクセッション番号

P08047

輸送温度

dry ice

関連するカテゴリー

詳細

105 kDa and 95 kDa
Every lot of the ChIPAb+ line of antibodies is individually validated for chromatin precipitation, in order to guarantee successful ChIP assays every time. Each antibody includes a control primer set for performance confirmation. Sp1 antibody and the negative control antibody (rabbit normal IgG) can be used to demonstrate that the Sp1 antibody is functionally validated in the precipitation of Sp1 associated chromatin.
The qPCR primers included flank the Sp1 binding site in human DHFR promoter.
Transcription factor SP1 can activate or repress transcription in response to physiological and pathological stimuli. Binds with high affinity to GC-rich motifs and regulates the expression of a large number of genes involved in a variety of processes such as cell growth, apoptosis, differentiation and immune responses. Highly regulated by post-translational modifications (phosphorylations, sumoylation, proteolytic cleavage, glycosylation and acetylation). Binds also the PDGFR-alpha G-box promoter. May have a role in modulating the cellular response to DNA damage. Implicated in chromatin remodeling. Plays a role in the recruitment of SMARCA4/BRG1 on the c-FOS promoter. Plays an essential role in the regulation of FE65 gene expression. In complex with ATF7IP, maintains telomerase activity in cancer cells by inducing TERT and TERC gene expression. Isoform 3 is a stronger activator of transcription than isoform 1. Positively regulates the transcription of the core clock component ARNTL/BMAL1.

免疫原

Full length human Sp1 protein

アプリケーション

Research Category
エピジェネティクス及び核内機能分子
Research Sub Category
エピジェネティクス
SP1 ChIP validated antibody & primer set including the ChIP-grade antibody & the specific control PCR primers used for chromatin immunoprecipitation of SP1.
Western Blot Analysis:
HeLa nuclear extract was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-Sp1 (0.2 μg/mL). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system (Please see figures).

生物化学的／生理学的作用

Sp1

包装

25 assays per kit, ~4μg per chromatin immunoprecipitation.

物理的形状

Protein A purified
Anti-Sp1 (Rabbit polyclonal IgG). 1 vial containing 100 μg of protein A purified IgG in 100 μL of 0.014 M phosphate buffer, pH 7.6, 0.175 M NaCl, 0.07% sodium azide and 30% glycerol. Store at -20°C. The Sp1 antibody is made against full length human Sp1 protein and can recognize Sp1, MW 105 kDa and 95 kDa, of human, mouse and rat origins.

Normal Rabbit IgG. One vial containing 125 μg of normal rabbit IgG in 125 μL. Store at -20°C.

ChIP primers DHFR. 1 vial containing 75 μL of 5 μM of each control primer specific for human dihydrofolate reductase (DHFR) promoter. Store at -20°C.
FOR: TCG CCT GCA CAA ATA GGG AC
REV: AGA ACG CGC GGT CAA GTT T
Format: Purified

調製ノート

1 year at -20°C from date of shipment.

アナリシスノート

Chromatin immunoprecipitation:
Sonicated chromatin prepared from 1 x 106 HeLa cells was subjected to chromatin immunoprecipitation using 4 μg of either the negative control antibody, Rabbit Normal IgG or Rabbit Anti-Sp1 and the Magna ChIPTM A kit (Cat.# 17-610) Rapid Protocol (2 hour IP). Successful enrichment of Sp1 associated DNA fragments was verified by qPCR using ChIP Primers DHFR flanking the human DHFR promoter that contains a Sp1 binding site (Please see figures).
Please refer to the EZ-Magna ChIP A (Cat.# 17-408)
or EZ-ChIP (Cat.# 17-371) kit protocols for
experimental details.
Control
Includes negative control rabbit IgG and control primers specific for human dihydrofolate reductase (DHFR) promoter.

法的情報

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

保管分類コード

10 - Combustible liquids

適用法令

試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。

毒物及び劇物取締法

キットコンポーネントの情報を参照してください

PRTR

キットコンポーネントの情報を参照してください

消防法

キットコンポーネントの情報を参照してください

労働安全衛生法名称等を表示すべき危険物及び有害物

キットコンポーネントの情報を参照してください

労働安全衛生法名称等を通知すべき危険物及び有害物

キットコンポーネントの情報を参照してください

カルタヘナ法

キットコンポーネントの情報を参照してください

Jan Code

キットコンポーネントの情報を参照してください

試験成績書（COA）

製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。

以前この製品を購入いただいたことがある場合

文書ライブラリで、最近購入した製品の文書を検索できます。

文書ライブラリにアクセスする

A feedback loop consisting of microRNA 23a/27a and the ?-like globin suppressors KLF3 and SP1 regulates globin gene expression.
Ma, Y; Wang, B; Jiang, F; Wang, D; Liu, H; Yan, Y; Dong, H; Wang, F; Gong, B; Zhu et al.
Molecular and cellular biology null
Epigenetic up-regulation of leukemia inhibitory factor (LIF) gene during the progression to breast cancer.
Shin, JE; Park, SH; Jang, YK
Molecules and Cells null
Paulisally Hau Yi Lo et al.
PloS one, 8(4), e61279-e61279 (2013-04-18)
Hepatitis C virus (HCV) infection is the major cause of hepatocellular carcinoma (HCC) in Japan. We previously identified the association of SNP rs2596542 in the 5' flanking region of the MHC class I polypeptide-related sequence A (MICA) gene with the
Silvia Salatino et al.
Molecular endocrinology (Baltimore, Md.), 30(7), 809-825 (2016-05-18)
The peroxisome proliferator-activated receptor-γ coactivator 1α (PGC-1α) coordinates the transcriptional network response to promote an improved endurance capacity in skeletal muscle, eg, by coactivating the estrogen-related receptor-α (ERRα) in the regulation of oxidative substrate metabolism. Despite a close functional relationship
Yuanyuan Zhao et al.
Molecular oncology, 12(3), 305-321 (2017-12-08)
Transforming growth factor-beta (TGF-β) functions as a potent proliferation inhibitor and apoptosis inducer in the early stages of breast cancer, yet promotes cancer aggressiveness in the advanced stages. The dual effect of TGF-β on cancer development is known as TGF-β
関連する文献をすべて表示

関連コンテンツ

Product Selection Guide - Antibodies, small molecule inhibitors, kits, assays and proteins for signaling research.

Signaling Product Guide: Antibodies, small molecule inhibitors, kits, assays and proteins for signaling research.

Advance your Epigenetics Research

Epigenetics describes heritable changes in gene expression caused by non-genetic mechanisms. Epigenetic regulation allows a cell to vary its response based on its biological and environmental contexts. Epigenetic changes can effect transcriptional and post-transcriptional regulation via mechanisms such as histone modification, chromatin and nucleosome remodeling, DNA methylation, and small and non-coding RNA-mediated regulation. These mechanisms, in cooperation with transcription factors and other nucleic acid-binding proteins, regulate gene expression. Epigenetic mechanisms of gene regulation impacts diverse areas of research—from agriculture to human health. Common epigenetic assays such as chromatin immunoprecipitation (ChIP) and RNA immunoprecipitation (RIP) rely on high quality antibodies that recognize specific epigenetic modifications for accurate results. EMD Millipore offers over 100 ChIPAb+™ and RIPAb+™ validated antibody kits that are quality tested on ChIP/RIP assays and are conveniently provided with control qPCR primers and negative control antibodies to ensure first time ChIP/RIP success.

Shaping Epigenetics Discovery - Epigenetics Product Selection Brochure

"Epigenetics describes heritable changes in gene expression caused by non-genetic mechanisms instead of by alterations in DNA sequence. These changes can be cell- or tissue-specific, and can be passed on to multiple generations. Epigenetic regulation enriches DNAbased information, allowing a cell to vary its response across diverse biological and environmental contexts. Although epigenetic mechanisms are primarily centered in the nucleus, these mechanisms can be induced by environmental signals such as hormones, nutrients, stress, and cellular damage, pointing to the involvement of cytoplasmic and extracellular factors in epigenetic regulation."

グローバルトレードアイテム番号

カタログ番号GTIN
17-60104053252733192

ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.

製品に関するお問い合わせはこちら（テクニカルサービス）