8.51072
6-Carboxyfluorescein
Novabiochem®
別名:
6-Carboxyfluorescein, 6-FAM
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この商品について
Quality Level
product line
Novabiochem®
assay
≥95% (TLC), ≥98.0% (HPLC)
form
powder
reaction suitability
reagent type: fluorescent-labelling reagent
manufacturer/tradename
Novabiochem®
application(s)
peptide synthesis
storage temp.
2-8°C
SMILES string
O1C3(c4c(cc(cc4)O)Oc5c3ccc(c5)O)c2c(ccc(c2)C(=O)O)C1=O
InChI
1S/C21H12O7/c22-11-2-5-14-17(8-11)27-18-9-12(23)3-6-15(18)21(14)16-7-10(19(24)25)1-4-13(16)20(26)28-21/h1-9,22-23H,(H,24,25)
InChI key
BZTDTCNHAFUJOG-UHFFFAOYSA-N
General description
This high purity fluorescent dye (λem494 nm, λex 518 nm) is supplied as a single isomer, which ensures production of labeled products of defined chemical structure, as well as greatly simplifying purification and characterization. FAM should be introduced as the last step in the synthesis to avoid formation of side-products, or protected by tritylation prior to acylation or hydrazine treatment [1].
Literature references
[1] R. Fischer, et al. (2003) Bioconjugate Chem., 14, 653.
Literature references
[1] R. Fischer, et al. (2003) Bioconjugate Chem., 14, 653.
Analysis Note
Color (visual): yellow to orange
Appearance of substance (visual): powder
Identity (IR): passes test
Purity (TLC (EtOAc/MeOH/H2O/DIPEA 60 : 30 : 10 : 3)): ≥ 95 %
Assay (HPLC, area%): ≥ 98.0 % (a/a)
To see the solvent systems used for TLC of Novabiochem® products please click here.
Appearance of substance (visual): powder
Identity (IR): passes test
Purity (TLC (EtOAc/MeOH/H2O/DIPEA 60 : 30 : 10 : 3)): ≥ 95 %
Assay (HPLC, area%): ≥ 98.0 % (a/a)
To see the solvent systems used for TLC of Novabiochem® products please click here.
Other Notes
Replaces: 01-63-0113
Legal Information
Novabiochem is a registered trademark of Merck KGaA, Darmstadt, Germany
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保管分類
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
試験成績書（COA）
製品のロット番号またはバッチ番号を入力して、分析証明書（COA）を検索してください。ロット番号およびバッチ番号は、製品のラベル上の「Lot」または「Batch」という表記の後に記載されています。
資料
Chromogenic and fluorogenic derivatives are invaluable tools for biochemistry, having numerous applications in enzymology, protein chemistry, immunology and histochemistry.
グローバルトレードアイテム番号
| カタログ番号 | GTIN |
|---|---|
| 8.51072.8100 | 04027269083656 |
| 8.51072.8025 | 04027269083649 |