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Merck

MHSTCMAG-70K

Millipore

MILLIPLEX® Mouse High Sensitivity T Cell Panel - Immunology Multiplex Assay

Simultaneous analyze low levels of cytokine and chemokine biomarker with the High Sensitivity Bead-Based Multiplex Assays using the Luminex technology, in mouse serum, plasma and cell culture samples.

別名:

Luminex® Mouse Immunology Panel, Millipore Mouse Immunology Panel, Mouse Immunology Protein Multiplex Assay

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この商品について

UNSPSCコード:
12161503
eCl@ss:
32161000
NACRES:
NA.47
テクニカルサービス
お困りのことがあれば、経験豊富なテクニカルサービスチームがお客様をサポートします。
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品質水準

交差性

mouse

メーカー/製品名

Milliplex®

assay range

accuracy: 82-102%
sensitivity: 0.26-11.14 pg/mL
standard curve range: 0.10-400 pg/mL
(IL-4)

standard curve range: 0.49-2,000 pg/mL
(IFNγ)

standard curve range: 0.49-2,000 pg/mL
(IL-17A)

standard curve range: 0.49-2,000 pg/mL
(IL-7)

standard curve range: 0.49-2,000 pg/mL
(TNFα)

standard curve range: 0.73-3,000 pg/mL
(KC/GROα/CXCL1)

standard curve range: 0.98-4,000 pg/mL
(IL-2)

standard curve range: 0.98-4,000 pg/mL
(IL-5)

standard curve range: 0.98-4,000 pg/mL
(IL-6)

standard curve range: 1.34-5,500 pg/mL
(IL-10)

standard curve range: 2.93-12,000 pg/mL
(IL-12 (p70))

standard curve range: 3.17-13,000 pg/mL
(LIX)

standard curve range: 3.42-14.000 pg/mL
(IL-13)

standard curve range: 3.91-16,000 pg/mL
(IL-1α)

standard curve range: 3.91-16,000 pg/mL
(IL-1β)

standard curve range: 3.91-16,000 pg/mL
(MCP-1/CCL2)

standard curve range: 6.1-25,000 pg/mL
(GM-CSF)

standard curve range: 7.32-30,000 pg/mL
(MIP-2/CXCL2)

inter-assay cv: <15%
intra-assay cv: <10%

テクニック

multiplexing: suitable

検出方法

fluorometric (Luminex xMAP)

輸送温度

ambient

詳細

Cytokines are immunomodulatory polypeptides that play key roles in both adaptive and innate immune responses. A generic term, “cytokines” includes myokines (produced by muscle cells), lymphokines (produced by activated Th cells), interleukins (acting as mediators between T cells), and chemokines (responsible for T-cell migration). One of the regulatory mechanisms of the immune system, cytokines act at the recognition, activation, or effector phases of an immune response, modulating the development and functional activities of the subtypes of T cells, B cells, and myeloid cells. Research involving cytokines plays a significant role in achieving a deeper understanding of the immune system and its multi-faceted response to most antigens, especially those responses that make up the inflammatory process.

Low levels of inflammation are involved in many clinical and sub-clinical disease states, such as autoimmune disease, cardiovascular disease, diabetes, neurological disorders, and cancer. Measuring picogram levels of cytokines, therefore, is critical for understanding the pathogenesis of these diseases.

Panel Type: Cytokines/Chemokines

アプリケーション

  • Analytes: GM-CSF, IFNγ, IL-1α, IL-1β, IL-2, IL-4, IL-5, IL-6, IL-7, IL-10, IL-12 (p70), IL-13, IL-17A, KC/CXCL1, LIX, MCP-1, MIP-2, TNF-α
  • Recommended Sample type: serum, plasma, tissue culture or other media
  • Recommended Sample dilution: neat or 1:2 diluted serum or plasma
  • Assay Run Time: Overnight
  • Research Category: Inflammation & Immunology
  • Research Subcategory: Immune Response Metabolic Disorders Chronic Inflammatory Disease Cardiovascular Disease

生物化学的/生理学的作用

Cross Reactivty
There is no detectable cross-reactivity within the panel.
Cross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.

特徴および利点

Design your multiplex kit by choosing available analytes within this panel.

調製ノート

Recommended storage for kit components is 2 - 8°C.

その他情報

Please contact Technical Service for linearity of dilution.
Sensitivity: Please see protocol for sensitivity of individual analytes.

法的情報

Luminex is a registered trademark of Luminex Corp
MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

ピクトグラム

Health hazardCorrosionExclamation markEnvironment

シグナルワード

Danger

危険有害性の分類

Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Skin Sens. 1 - STOT RE 2

ターゲットの組織

Respiratory Tract

保管分類コード

10 - Combustible liquids


適用法令

試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。

毒物及び劇物取締法

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PRTR

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消防法

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労働安全衛生法名称等を表示すべき危険物及び有害物

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労働安全衛生法名称等を通知すべき危険物及び有害物

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カルタヘナ法

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Jan Code

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試験成績書(COA)

製品のロット番号・バッチ番号を入力して、試験成績書(COA) を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。

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関連コンテンツ

The MILLIPLEX® Mouse High Sensitivity T Cell Multiplex Panel is a powerful tool for cytokine profiling in biological samples. Uncover details on how this panel was evaluated for studying low-level cytokine expression and also quantifying multiple cytokine secretion levels simultaneously.

MILLIPLEX® Mouse High Sensitivity T Cell Multiplex Panelは、生体サンプルにおけるサイトカインプロファイリングのための強力なツールです。低レベルのサイトカイン発現を調べ、複数のサイトカインを同時に定量するための、このパネルの評価方法について詳しく紹介します。

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Increasing evidence indicates that inflammatory responses may influence brain neurochemical pathways, inducing depressive-like behaviors. Ultrasound stimulation (US) is a promising non-invasive treatment for neuropsychiatric diseases. We investigated whether US can suppress inflammation and improve depressive-like behaviors. Mice were intraperitoneally injected
Robert A Kazmierczak et al.
Oncotarget, 11(44), 3943-3958 (2020-11-21)
Conventional cancer chemotherapies are not fully efficacious and do not target tumors, leading to significant treatment-related morbidities. A number of genetically attenuated cancer-targeting bacteria are being developed to safely target tumors in vivo. Here we report the toxicological, tumor-targeting, and
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Frontiers in cellular and infection microbiology, 11, 618265-618265 (2021-04-06)
Aging induced chronic systemic inflammatory response is an important risk factor for atherosclerosis (AS) development; however, the detailed mechanism is yet to be elucidated. To explore the underlying mechanism of how aging aggravates AS advancement. A young (five-week-old, YM) and