Anti-phospho-ITCH (pT222) Antibody

ITCH is an E3 ubiquitin-protein ligase that accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. ITCH regulates the transcriptional activity of several transcription factors including the hematopoietic transcription factor NF-E2, and may play an important role in the regulation of immune response. ITCH down-regulates Epstein-Barr virus LMP2A activity in B cell signaling, and also interacts with NOTCH1, OCLN, JUN, and JUNB. ITCH has a wide tissue distribution and is localized within the nucleus and cytoplasm. Two ITCH splice variants have been described; an approximately 103 kDa form as well as a truncated form (~98 kDa) lacking 42 amino acids in the N-terminal half of the protein. ITCH contains a MAP Kinase binding site and its activity is modulated by phosphorylation at multiple threonine residues by JNK1.

Synthetic peptide corresponding to a portion of human ITCH encompassing the phosphorylated Threonine at position 222 (PPPpTPRRP).

Research Category
Protein Trafficking

Research Sub Category
Ubiquitin & Ubiquitin Metabolism

This Anti-phospho-ITCH (pT222) Antibody is validated for use in DB, WB for the detection of phospho-ITCH (pT222).

Western Blot: Recommended Dilution 1 - 5 μg/mL
Dot Blot

Human ITCH phosphorylated on threonine 222 (T222).

Affinity purified immunoglobulin. Liquid in Tris-citrate/phosphate buffer, pH 7-8, containing 0.1% sodium azide.

Maintain at 2-8°C for up to 12 months from date of receipt

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

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