Anti-Recoverin Antibody

Recoverin is a calcium-binding protein expressed in retinal photoreceptors and midget cone bipolar cells. It appears to delay the termination of the phototransduction cascade by blocking the phosphorylation of photoexcited rhodopsin.

26 kDa

Recombinant human recoverin.

Detect Recoverin using this Anti-Recoverin Antibody validated for use in IC, IH, IH(P) & WB.

Research Category
Neuroscience

Research Sub Category
Sensory & PNS

Western Blot Analysis:
1:1,000-1:5,000 dilution using ECL.

Immunohistochemistry:
1:1,000 (2 hours at room temperature or overnight at 2-8ºC) on frozen and paraffin embedded tissue sections. Suggested fixative is 4% paraformaldehyde or 3% paraformaldehyde with 0.1% glutaraldehyde. Suggested permeabilization solution is 0.1% Triton X-100. Suggested blocking buffer is 0.05 M Tris saline, pH 7.4 containing 2.5% BSA, 0.1% triton and 0.05% sodium azide.

Optimal working dilutions must be determined by the end user.

Other species have not been tested.

Recognizes recoverin.

Rabbit polyclonal serum. Contains no preservative.

Unpurified

Stable for 1 year at -20ºC from date of receipt.

Control
POSITIVE CONTROL: Recoverin is found in photoreceptors and midget cone bipolar cells in retina and pinealocytes in pineal gland.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Replaces: AB5431P

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