ABT146
Anti-Occludin Antibody
from rabbit, purified by affinity chromatography
別名:
Occludin
製品名
Anti-Occludin Antibody, from rabbit, purified by affinity chromatography
biological source
rabbit
conjugate
unconjugated
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
purified by
affinity chromatography
species reactivity
human
species reactivity (predicted by homology)
bovine (based on 100% sequence homology), mouse (based on 100% sequence homology), porcine (based on 100% sequence homology), canine (based on 100% sequence homology), primate (based on 100% sequence homology), rat (based on 100% sequence homology), equine (based on 100% sequence homology)
packaging
antibody small pack of 25 μg
technique(s)
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
ambient
target post-translational modification
unmodified
Quality Level
Gene Information
human ... OCLN(4950), OCLN(100506658)
Analysis Note
Control
Human kidney tissue lysate
Human kidney tissue lysate
Evaluated by Western Blot in human kidney tissue lysate.
Western Blot Analysis: 0.2 µg/mL of this antibody detected Occludin in 10 µg of human kidney tissue lysate.
Western Blot Analysis: 0.2 µg/mL of this antibody detected Occludin in 10 µg of human kidney tissue lysate.
Application
Research Category
Cell Structure
Cell Structure
Research Sub Category
ECM Proteins
ECM Proteins
This Anti-Occludin Antibody is validated for use in Western Blotting for the detection of Occludin.
Biochem/physiol Actions
This antibody recognizes the N-terminus of Occludin.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description
Occludin is a 65-kDa integral plasma-membrane protein located at the tight junctions. It is required for cytokine-induced regulation of the tight junction paracellular permeability barrier. Occludin is believed to prevent the transport of macromolecules through the tight junction. Encoded by the OLCN gene, occludin spans the plasma membrane four times, forming two extracellular loops and exposing its NH2 and COOH terminus to the cytosol. This cytoplasmic tail binds directly to the ZO family of proteins, which link the protein complex to the actin cytoskeleton. Phosphorylation/dephosphorylation plays a major role in regulation of occludin and tight junctions. Expression of occludin is required for infection of liver cells by hepatitis C virus (HCV).
~63 kDa observed. The calculated molecular weight is 63 kDa, however Occludin has been shown ranging from ~55 to ~65 kDa in western blots (Florian, P., et al. (2003). Biochemical and Biophysical Research Communications. 304:5-10).
Immunogen
Epitope: N-terminus
KLH-conjugated linear peptide corresponding to the N-terminus of human Occludin.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Physical form
Affinity purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
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試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
Hengameh Chloé Mirsepasi-Lauridsen et al.
Clinical and translational gastroenterology, 7, e149-e149 (2016-03-05)
The potential of Escherichia coli (E. coli) isolated from inflammatory bowel disease (IBD) patients to damage the integrity of the intestinal epithelium was investigated. E. coli strains isolated from patients with ulcerative colitis (UC) and healthy controls were tested for
Duobin Zhang et al.
Journal of leukocyte biology, 111(5), 953-966 (2021-09-30)
Ischemic stroke (IS) can cause disability and death, and microglia as the immune component of the CNS can release inflammatory factors and participate in blood-brain barrier (BBB) dysfunction. This study aimed to investigate the effects of long noncoding RNA (lncRNA)
Diana Duarte Lobo et al.
Acta neuropathologica communications, 8(1), 152-152 (2020-09-02)
Blood-brain barrier (BBB) disruption is a common feature in neurodegenerative diseases. However, BBB integrity has not been assessed in spinocerebellar ataxias (SCAs) such as Machado-Joseph disease/SCA type 3 (MJD/SCA3), a genetic disorder, triggered by polyglutamine-expanded ataxin-3. To investigate that, BBB
Kristin Elfers et al.
PloS one, 11(4), e0154311-e0154311 (2016-04-28)
Diets fed to ruminants should contain nitrogen (N) as low as possible to reduce feed costs and environmental pollution. Though possessing effective N-recycling mechanisms to maintain the N supply for rumen microbial protein synthesis and hence protein supply for the
Maj Schneider Thomsen et al.
PloS one, 16(3), e0236770-e0236770 (2021-03-13)
The blood-brain barrier (BBB) is formed by brain capillary endothelial cells (BECs) supported by pericytes and astrocytes. The BBB maintains homeostasis and protects the brain against toxic substances circulating in the blood, meaning that only a few drugs can pass
グローバルトレードアイテム番号
| カタログ番号 | GTIN |
|---|---|
| ABT146 | 04053252819520 |
| ABT146-25UG | 04054839350320 |
ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.
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