CBL171
Anti-α-Smooth Muscle Actin (ACTA2) Antibody,
CHEMICON®, mouse monoclonal, ASM-1
別名:
Alpha-actin-2
製品名
Anti-Actin Antibody, smooth muscle, clone ASM-1, clone ASM-1, Chemicon®, from mouse
biological source
mouse
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
ASM-1, monoclonal
species reactivity
chicken, mouse, horse, rat, human, bovine
manufacturer/tradename
Chemicon®
technique(s)
immunofluorescence: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable
isotype
IgG2a
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
unmodified
Quality Level
Gene Information
human ... ACTA2(59)
Analysis Note
Control
Positive reaction using stress fibers of smooth muscle-derived cells and some smooth muscle subtype fibroblasts.
Positive reaction using stress fibers of smooth muscle-derived cells and some smooth muscle subtype fibroblasts.
Evaluated by Western Blot on HUVEC lysates.
Western Blot Analysis:
1:1000 dilution of this antibody detected ACTIN, SM MUSC on 10 μg of HUVEC lysates.
Western Blot Analysis:
1:1000 dilution of this antibody detected ACTIN, SM MUSC on 10 μg of HUVEC lysates.
Application
Anti-Actin Antibody, smooth muscle, clone ASM-1 detects level of Actin & has been published & validated for use in IF, IH, IH(P) & WB.
Immunohistochemistry:
1:2000 dilution of a previous lot was used on frozen and formalin-fixed, paraffin-embedded tissues; protease pretreatment is recommended for paraffin-embedded sections.
Immunofluorescence:
A previous lot of this antibody was used on Immunofluoresence.
Optimal working dilutions must be determined by the end user.
1:2000 dilution of a previous lot was used on frozen and formalin-fixed, paraffin-embedded tissues; protease pretreatment is recommended for paraffin-embedded sections.
Immunofluorescence:
A previous lot of this antibody was used on Immunofluoresence.
Optimal working dilutions must be determined by the end user.
Research Category
Cell Structure
Cell Structure
Research Sub Category
Cytoskeletal Signaling
Cytoskeletal Signaling
Biochem/physiol Actions
Expected to cross-react with bovine, equine and chicken. Reactivity with other species has not been confirmed.
This antibody is specific for the alpha-smooth-muscle isoform of actin (MW 43 kDa).
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description
43 kDa
ASM-1 represents an excellent marker for myogenic soft tissue tumors and smooth muscle differentiation. This antibody reacts with many types of smooth muscle cells, such as those present in vascular walls, intestinal muscularis mucosae and propria, myometrium, stroma of various tissues, and is also positive for myoepithelial cells of various glands, notably salivary and mammary gland. Myogenic soft tissues detected include leiomyosarcomas, leiomyomas, and certain stromal cells surrounding infiltrating ductal carcinoma of the breast.
Immunogen
Epitope: N-terminus
Synthetic peptide corresponding to the ten N-terminal amino acids of the alpha-smooth muscle isoform of actin.
Other Notes
Replaces: 04-1040
Physical form
Format: Purified
Protein A purified
Purified mouse monoclonal IgG2a presented as lyophilized material. Reconstitute in 1 mL of of distilled water.
Final solution is PBS, pH 7.4, containing 0.5% BSA and 0.09% sodium azide.
Final solution is PBS, pH 7.4, containing 0.5% BSA and 0.09% sodium azide.
Preparation Note
Stable for 1 year at -20°C in undiluted aliquots from date of receipt.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
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signalword
Warning
hcodes
Hazard Classifications
Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Aquatic Chronic 3
保管分類
11 - Combustible Solids
wgk
WGK 3
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
Reprogramming LCLs to iPSCs Results in Recovery of Donor-Specific Gene Expression Signature.
Thomas, SM; Kagan, C; Pavlovic, BJ; Burnett, J; Patterson, K; Pritchard, JK; Gilad, Y
PLoS Genetics null
Localization of angiopoietin-1 and Tie2 immunoreactivity in rodent ependyma and adjacent blood vessels suggests functional relationships.
Horton, BN; Solanki, RB; Rajneesh, KF; Kulesza, P; Ardelt, AA
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society null
Junfeng Zhang et al.
PloS one, 8(12), e83294-e83294 (2013-12-19)
Endothelial progenitor cells (EPCs) are capable of proliferating and differentiating into mature endothelial cells, and they have been considered as potential candidates for coronary heart disease therapy. However, the transition of EPCs to mesenchymal cells is not fully understood. This
Over-expression of CD9 does not affect in vivo tumorigenic or metastatic properties of human prostate cancer cells.
Valerii Zvieriev, Jia-Chi Wang, Mario Chevrette
Biochemical and biophysical research communications null
V Zvereff et al.
British journal of cancer, 97(7), 941-948 (2007-09-13)
CD9, a member of the tetraspanin family of proteins, is involved in a variety of cellular interactions with many other proteins and molecules. Although CD9 has been implicated in cell fusion, migration and cancer progression, the detailed function of this
グローバルトレードアイテム番号
| カタログ番号 | GTIN |
|---|---|
| CBL171 | 04053252589379 |
ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.
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