CP70
Anti-Fibronectin (Ab-3) Mouse mAb (FBN11)
liquid, clone FBN11, Calbiochem®
製品名
Anti-Fibronectin (Ab-3) Mouse mAb (FBN11), liquid, clone FBN11, Calbiochem®
biological source
mouse
antibody form
purified antibody
antibody product type
primary antibodies
clone
FBN11, monoclonal
form
liquid
contains
≤0.1% sodium azide as preservative
species reactivity
rat, human, mouse
manufacturer/tradename
Calbiochem®
storage condition
do not freeze
dilution
(Frozen Sections (0.5-1 µg/mL)
Immunoblotting (0.5-1 µg/mL)
Immunofluorescence
Immunoprecipitation (2 µg/mg protein lysate)
Paraffin Sections (0.5-1 µg/mL, heat pre-treatment required))
isotype
IgG1
shipped in
wet ice
storage temp.
2-8°C
target post-translational modification
unmodified
Quality Level
Gene Information
human ... FN1(2335)
mouse ... Fn1(14268)
rat ... Fn1(25661)
Analysis Note
Positive Control
SW 156 cells or normal kidney tissue
SW 156 cells or normal kidney tissue
Application
Frozen Sections (0.5-1 µg/ml)
Immunoblotting (0.5-1 µg/ml)
Immunofluorescence (see comments)
Immunoprecipitation (2 µg/mg protein lysate)
Paraffin Sections (0.5-1 µg/ml, heat pre-treatment required)
Immunoblotting (0.5-1 µg/ml)
Immunofluorescence (see comments)
Immunoprecipitation (2 µg/mg protein lysate)
Paraffin Sections (0.5-1 µg/ml, heat pre-treatment required)
Disclaimer
Toxicity: Standard Handling (A)
General description
Anti-Fibronectin (Ab-3), mouse monoclonal, clone FBN11, recognizes dimeric fibronectin in SWI56 cells and normal kidney. It is validated for use in WB, IF, IP & IHC (frozen and paraffin sections).
Protein G purified mouse monoclonal antibody. Recognizes the ~440 kDa disulfide-linked, dimeric fibronectin.
Recognizes the ~440 kDa disulfide-linked, dimeric fibronectin in SWI56 cells and normal kidney tissue.
Immunogen
recombinant human fibronectin
Other Notes
Clark, R.A. 2001. Ann. N.Y. Acad. Sci.936, 355.
Armstrong, P.B. and Armstrong, M.T. 2000. Biochim. Biophys. Acta1470, O9.
Barilla, M.L. and Carsons, S.E. 2000. Semin. Arthritis Rheum.29, 252.
Yamada, K.M. 1989. In Fibronectin, Mosher, D.F., ed., p. 47.
Rouslahti, E. and Pierschbacher, M.D. 1986. Cell44, 517.
Pytela, R., et al. 1985. Cell40, 191.
Armstrong, P.B. and Armstrong, M.T. 2000. Biochim. Biophys. Acta1470, O9.
Barilla, M.L. and Carsons, S.E. 2000. Semin. Arthritis Rheum.29, 252.
Yamada, K.M. 1989. In Fibronectin, Mosher, D.F., ed., p. 47.
Rouslahti, E. and Pierschbacher, M.D. 1986. Cell44, 517.
Pytela, R., et al. 1985. Cell40, 191.
Exhibits staining of the connective tissue matrix when used in immunohistochemistry. This antibody is suitable for both reduced and non-reduced protein by immunoblotting. For staining of formalin-fixed tissues, boil in 10 mM citrate buffer, pH 6.0, for 10-20 min, followed by cooling at room temperature for 20 min. This antibody has also been reported to work for immunofluorescence. Antibody should be titrated for optimal results in individual systems.
Packaging
Please refer to vial label for lot-specific concentration.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
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保管分類
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
R Schaal-Jensen et al.
Journal of thrombosis and haemostasis : JTH, 5(10), 2070-2078 (2007-07-26)
In cynomolgus monkeys, suprapharmacological doses of clotting recombinant factor XIII (rFXIII) cause a generalized coagulopathy, associated with formation of circulating high molecular weight protein complexes (HMEX). HMEX consist of plasma protein substrates cross-linked by FXIII transglutaminase activity. To characterize HMEX
Ismaïl Hendaoui et al.
Matrix biology : journal of the International Society for Matrix Biology, 40, 46-53 (2014-09-10)
Whisker follicles have multiple stem cell niches, including epidermal stem cells in the bulge as well as neural crest-derived stem cells and mast cell progenitors in the trabecular region. The neural crest-derived stem cells are a pool of melanocyte precursors.
Yu-Jen Chiu et al.
International journal of oncology, 52(5), 1465-1478 (2018-03-24)
Osteosarcoma is the most common primary malignancy of the bone and is characterized by local invasion and distant metastasis. Over the past 20 years, long-term outcomes have reached a plateau even with aggressive therapy. Overexpression of insulin-like growth factor 1 receptor
Nicole Vincent Jordan et al.
Molecular and cellular biology, 33(15), 3011-3025 (2013-05-30)
We previously identified a gene signature predicted to regulate the epithelial-mesenchymal transition (EMT) in both epithelial tissue stem cells and breast cancer cells. A phenotypic RNA interference (RNAi) screen identified the genes within this 140-gene signature that promoted the conversion
グローバルトレードアイテム番号
| カタログ番号 | GTIN |
|---|---|
| CP70-100UGCN | 04055977221015 |
ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.
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