CP74
Anti-Vinculin Mouse mAb (V284 or VLN01)
liquid, Calbiochem®, from mouse
別名:
Anti-Vinculin antibody
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この商品について
NACRES:
NA.41
UNSPSC Code:
12352203
Clone:
V284, monoclonal
VLN01, monoclonal
VLN01, monoclonal
Species reactivity:
human
Application:
Citations:
21
製品名
Anti-Vinculin Mouse mAb (V284 or VLN01), liquid, Calbiochem®, from mouse
biological source
mouse
antibody form
purified antibody
antibody product type
primary antibodies
clone
V284, monoclonal
VLN01, monoclonal
form
liquid
contains
≤0.1% sodium azide as preservative
species reactivity
human
manufacturer/tradename
Calbiochem®
storage condition
do not freeze
dilution
(Immunoblotting (1 µg/mL, chemiluminescence)
Immunoprecipitation (2 µg/mL lysate)
Paraffin Sections (4-8 µg/mL, heat pre-treatment required))
isotype
IgG1
shipped in
wet ice
storage temp.
2-8°C
target post-translational modification
unmodified
Quality Level
Gene Information
human ... VCL(7414)
Analysis Note
Negative Control
Normal mouse serum
Normal mouse serum
Positive Control
Skin cells or tissue
Skin cells or tissue
Application
Immunoblotting (1 g/ml, chemiluminescence)
Immunoprecipitation (2 g/ml lysate)
Paraffin Sections (4-8 g/ml, heat pre-treatment required)
Disclaimer
Toxicity: Standard Handling (A)
General description
Protein G purified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with mouse myeloma SP2/0 cells. Recognizes the ~130 (vinculin) and ~150 kDa (meta-vinculin) proteins.
Recognizes the ~130 (vinculin) and ~150 kDa (meta-vinculin) proteins in skin tissue.
This Anti-Vinculin Mouse mAb (V284 or VLN01) is validated for use in Immunoblotting, Immunoprecipitation, Paraffin Sections for the detection of Vinculin.
Immunogen
Human
semi-purified, human vinculin
Other Notes
Palovuori, R., and Eskelinen, S. 2000 Eur. J. Cell Biol.79, 961.
Bakolitsa, C., et al. 1999 Cell99, 603.
Rudiger, M., et al. 1998 Bioessays20, 733
Tidball, J.G., et al. 1999 J. Biol. Chem.274, 33155
Goldmann, W.H., et al. 1996 J. Muscle Res. Cell Motil.17, 1.
Asijee, G.M., et al. 1990 Eur. J. Biochemistry189, 131
Otto, J.J. 1990 Cell Motil. Cytoskeleton16, 1.
Bakolitsa, C., et al. 1999 Cell99, 603.
Rudiger, M., et al. 1998 Bioessays20, 733
Tidball, J.G., et al. 1999 J. Biol. Chem.274, 33155
Goldmann, W.H., et al. 1996 J. Muscle Res. Cell Motil.17, 1.
Asijee, G.M., et al. 1990 Eur. J. Biochemistry189, 131
Otto, J.J. 1990 Cell Motil. Cytoskeleton16, 1.
This antibody is useful for studies of the role of vinculin in platelet activation. Vinculin (Ab-1) exhibits membrane and cytoplasmic staining when used in immunohistochemistry. For staining of formalin-fixed paraffin sections, boil in 10 mM citrate buffer, pH 6.0, for 10-20 min, followed by cooling at room temperature for 20 min. Antibody should be titrated for optimal results in individual systems.
Packaging
Please refer to vial label for lot-specific concentration.
Physical form
In 10 mM PBS, 0.2% BSA, pH 7.4.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
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保管分類
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
CP74-UG: + CP74-100UG:
jan
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
The specific role of pRb in p16 (INK4A) -mediated arrest of normal and malignant human breast cells.
Alexey V Bazarov et al.
Cell cycle (Georgetown, Tex.), 11(5), 1008-1013 (2012-02-16)
RB family proteins pRb, p107 and p130 have similar structures and overlapping functions, enabling cell cycle arrest and cellular senescence. pRb, but not p107 or p130, is frequently mutated in human malignancies. In human fibroblasts acutely exposed to oncogenic ras
Tim Lahm et al.
American journal of physiology. Lung cellular and molecular physiology, 311(2), L375-L388 (2016-06-12)
17β-Estradiol (E2) exerts protective effects on right ventricular (RV) function in pulmonary arterial hypertension (PAH). Since acute exercise-induced increases in afterload may lead to RV dysfunction in PAH, we sought to determine whether E2 allows for superior RV adaptation after
Nitric Oxide Circumvents Virus-Mediated Metabolic Regulation during Human Cytomegalovirus Infection.
Rebekah L Mokry et al.
mBio, 11(6) (2020-12-17)
Nitric oxide is a versatile and critical effector molecule that can modulate many cellular functions. Although recognized as a regulator of infections, the inhibitory mechanism of nitric oxide against human cytomegalovirus (HCMV) replication remains elusive. We demonstrate that nitric oxide
Gesche Frohwitter et al.
Oncology letters, 12(1), 107-113 (2016-06-28)
Squamous cell carcinoma (SCC) of the oral cavity is a morphological heterogeneous disease. Various cytokeratin (CK) expression patterns with different prognostic values have been described, but little is known concerning the underlying biological cell mechanisms. Therefore, the present study investigated
Vaibhav Sidarala et al.
Nature communications, 13(1), 2340-2340 (2022-04-30)
The dynamin-like GTPases Mitofusin 1 and 2 (Mfn1 and Mfn2) are essential for mitochondrial function, which has been principally attributed to their regulation of fission/fusion dynamics. Here, we report that Mfn1 and 2 are critical for glucose-stimulated insulin secretion (GSIS)
グローバルトレードアイテム番号
| カタログ番号 | GTIN |
|---|---|
| 05-655 | 04053252744693 |
| CP74-100UGCN | 04055977221022 |
ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.
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