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Merck

DP02

Anti-SV40 T Antigen (Ab-2) Mouse mAb (PAb416)

liquid, clone PAb416, Calbiochem®

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この商品について

NACRES:
NA.41
UNSPSC Code:
12352203
Clone:
PAb416, monoclonal
Species reactivity:
SV40-infected cells
Application:
—
Citations:
15

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biological source

mouse

antibody form

purified antibody

antibody product type

primary antibodies

clone

PAb416, monoclonal

form

liquid

contains

≤0.1% sodium azide as preservative

species reactivity

SV40-infected cells

manufacturer/tradename

Calbiochem®

storage condition

do not freeze

dilution

(Affinity Purification
Immunoblotting (1-5 µg/mL)
Immunofluorescence (1-5 µg/mL)
Immunoprecipitation (1-5 µg antibody)
Paraffin Sections )

isotype

IgG2a

shipped in

wet ice

Quality Level

100

storage temp.

2-8°C

target post-translational modification

unmodified

General description

Purified mouse monoclonal antibody generated by immunizing mice with the specified immunogen and fusing splenocytes with NS-1 mouse myeloma cells (see application references). Recognizes the ~94 kDa SV40 large T antigen.
Recognizes ~94 kDa SV40 large T antigen in SV80 and SV-T2 cells.
SV40 T Ag (Ab-2) is a mouse monoclonal antibody with specificity for antigenic determinants unique to the SV40 large T antigen and non-reactive with SV40 small T antigen. Both antigens are encoded by the early region of the SV40 genome. The large T antigen binds DNA, and complexes with a 53,000 dalton cellular transforming protein, p53, which is required for initiation of viral DNA replication during lytic growth. In addition the large T antigen binds DNA polymerase and the transcription factor AP-2 and forms a specific complex with the p105 product of the retinoblastoma susceptibility gene.
This Anti-SV40 T Antigen (Ab-2) Mouse mAb (PAb416) is validated for use in Affinity Purification, Immunoblotting, IF, IP, Paraffin Sections for the detection of SV40 T Antigen (Ab-2).

Immunogen

Epitope: Within amino acids 83-128
purified SV40 large T-antigen

Application

Affinity Purification (use Cat. No. DP02A)

Immunoblotting (1-5 µg/ml, see application references)

Immunofluorescence (1-5 µg/ml)

Immunoprecipitation (1-5 µg antibody, use Cat. No. DP02A)

Paraffin Sections (see application references)

Packaging

Please refer to vial label for lot-specific concentration.

Physical form

In 50 mM sodium phosphate buffer, 0.2% gelatin, pH 7.5.

Analysis Note

Negative Control
3T3 cells
Positive Control
SV80 or SV-T2 cells

Other Notes

DeCaprio, J.A., et al. 1988. Cell54, 275.
Whyte, P., et al. 1988. Nature334, 124.
Mitchell, P.J., et al. 1987. Cell50, 847.
Dixon, R.A.F. and Nathans, D., 1985. J. Virol.53, 1001.
Simanis, V. and Lane, D.P. 1985. Virol.144, 88.
Mann, R.S. and Carroll, R.B. 1984. Virology138, 379.
Sarnow, P., et al. 1982. Cell28, 387.
Crawford, L.V., et al. 1981. Proc. Natl. Acad. Sci. USA78, 41.
Lane, D.P. and Crawford, L.V. 1979. Nature278, 261.
Carroll, R.B., et al. 1974. Proc. Natl. Acad. Sci. USA71, 3754.
Tooze, J. 1973. Cold Spring Harbor, New York.
Tegtmeyer, P. 1972. J. Virol.10, 591.
The agarose conjugate (Cat. No. DP02A) is also available and suitable for affinity purification and immunoprecipitation. Antibody should be titrated for optimal results in individual systems.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

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保管分類

12 - Non Combustible Liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

適用法令

試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。

DP02-1MG: + DP02-200UG:

jan

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Archives of pathology & laboratory medicine, 142(9), e2-e202 (2018-09-13)
and case study poster sessions will be conducted during the 2018 College of American Pathologists Annual Meeting (CAP18), which is scheduled for October 20 to 24, 2018. The meeting will take place at the Hyatt Regency, Chicago, Illinois. The poster
Tobias May et al.
Methods in molecular medicine, 140, 1-15 (2007-12-19)
Cell therapies rely on the implantation of well-characterized functional cells with defined properties. Often, the cells of interest do not proliferate in vitro and thus cannot be expanded to the amount needed for characterization, purification, manipulation, or cloning. Here, we
B K Kleinschmidt-DeMasters et al.
Journal of neuropathology and experimental neurology, 71(7), 604-617 (2012-06-20)
The use of natalizumab to treat multiple sclerosis (MS) has been associated with the development of progressive multifocal leukoencephalopathy (PML), with 242 PML cases reported as of May 3, 2012. Fortunately, rapid withdrawal of the drug and administration of plasma
V Krump-Konvalinkova et al.
Laboratory investigation; a journal of technical methods and pathology, 81(12), 1717-1727 (2001-12-14)
The limited lifespan of human microvascular endothelial cells in cell culture represents a major obstacle for the study of microvascular pathobiology. To date, no endothelial cell line is available that demonstrates all of the fundamental characteristics of microvascular endothelial cells.
S Bialasiewicz et al.
Transplant infectious disease : an official journal of the Transplantation Society, 15(3), 283-289 (2013-04-05)
BK virus (BKV) is an ubiquitous human polyomavirus that establishes latency in urothelium. BKV is known to re-activate in immunosuppressed individuals, and is an increasingly important cause of nephropathy and graft loss in kidney transplant recipients. Animal studies have demonstrated
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グローバルトレードアイテム番号

カタログ番号GTIN
DP02-200UGCN07790788053734

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