MAB3448
Anti-Heterochromatin Protein-1 β Antibody, clone 1MOD-1A9
ascites fluid, clone 1MOD-1A9, Chemicon®
別名:
HP1b
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この商品について
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
1MOD-1A9, monoclonal
Application:
ELISA, ICC, IHC, IP, WB
Citations:
84
biological source
mouse
conjugate
unconjugated
antibody form
ascites fluid
antibody product type
primary antibodies
clone
1MOD-1A9, monoclonal
species reactivity
mouse, human
manufacturer/tradename
Chemicon®
technique(s)
ELISA: suitable, immunocytochemistry: suitable, immunohistochemistry: suitable, immunoprecipitation (IP): suitable, western blot: suitable
isotype
IgG1
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
unmodified
Quality Level
Gene Information
human ... CBX1(10951)
General description
28 kDa
Chromatin assembly factor-1 (CAF-1) is a multisubunit protein complex that comprises three polypeptide subunits known as p150, p60, and p48. CAF-1 is a nucleosome assembly factor that deposits newly synthesized and acetylated histones H3/H4 into nascent chromatin during DNA replication.
Heterochromatin is characterized as densely coiled chromatin that generally replicates late during S phase, has a low gene density, and contains large blocks of repetitive DNA that is relatively inaccessible to DNA-modifying reagents. In late S phase, p150 directly associates with heterochromatin associated proteins 1 (HP1α, HP1β and HP1γ). As cells prepare for mitosis, CAF-1 p150 and some HP1 progressively dissociate from heterochromatin, coinciding with the phosphorylation of histone H3. The HP1 proteins reassociate with chromatin at the end of mitosis, as histone H3 is dephosphorylated.
Heterochromatin is characterized as densely coiled chromatin that generally replicates late during S phase, has a low gene density, and contains large blocks of repetitive DNA that is relatively inaccessible to DNA-modifying reagents. In late S phase, p150 directly associates with heterochromatin associated proteins 1 (HP1α, HP1β and HP1γ). As cells prepare for mitosis, CAF-1 p150 and some HP1 progressively dissociate from heterochromatin, coinciding with the phosphorylation of histone H3. The HP1 proteins reassociate with chromatin at the end of mitosis, as histone H3 is dephosphorylated.
Immunogen
Recombinant mouse HP1beta.
Application
Use Anti-Heterochromatin Protein-1 β Antibody, clone 1MOD-1A9 (Mouse Monoclonal Antibody) validated in ELISA, ICC, IHC, IP, WB to detect Heterochromatin Protein-1 β also known as HP1b.
Western blot: 1:500-1:5,000
Immunofluorescence: 1:500-1:5,000
Immunohistochemistry: 1:500-1:5,000
Immunoprecipitation: 1:500 - 1:5,000
ELISA: 1:500-1:5,000
Optimal working dilutions must be determined by the end user.
Immunofluorescence: 1:500-1:5,000
Immunohistochemistry: 1:500-1:5,000
Immunoprecipitation: 1:500 - 1:5,000
ELISA: 1:500-1:5,000
Optimal working dilutions must be determined by the end user.
Biochem/physiol Actions
Reacts with Heterochromatin protein-1 b (HP1b).
Analysis Note
Control
HeLa, 293 or NIH/3T3 cell lysates
HeLa, 293 or NIH/3T3 cell lysates
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
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wgk
WGK 1
保管分類
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
MAB3448:
jan
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
Structural differences in centromeric heterochromatin are spatially reconciled on fertilisation in the mouse zygote.
Aline V Probst, Fatima Santos, Wolf Reik, Genevieve Almouzni, Wendy Dean
Chromosoma null
The chemokine growth-regulated oncogene 1 (Gro-1) links RAS signaling to the senescence of stromal fibroblasts and ovarian tumorigenesis.
Yang, G; Rosen, DG; Zhang, Z; Bast, RC; Mills, GB; Colacino, JA; Mercado-Uribe, I; Liu, J
Proceedings of the National Academy of Sciences of the USA null
Epigenetic regulation of myogenic gene expression by heterochromatin protein 1 alpha.
Sdek, P; Oyama, K; Angelis, E; Chan, SS; Schenke-Layland, K; MacLellan, WR
Testing null
Jmjd1a and Jmjd2c histone H3 Lys 9 demethylases regulate self-renewal in embryonic stem cells.
Loh, YH; Zhang, W; Chen, X; George, J; Ng, HH
Genes & Development null
Essential role of DPPA3 for chromatin condensation in mouse oocytogenesis.
Liu, YJ; Nakamura, T; Nakano, T
Biology of reproduction null
グローバルトレードアイテム番号
| カタログ番号 | GTIN |
|---|---|
| MAB3448 | 04053252264900 |
ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.
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