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Merck

MAB380

Anti-β-Tubulin III Antibody

CHEMICON®, mouse monoclonal, 5H1

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この商品について

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

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製品名

Anti-Tubulin β Antibody, clone 5H1, ascites fluid, clone 5H1, Chemicon®

biological source

mouse

antibody form

ascites fluid

antibody product type

primary antibodies

clone

5H1, monoclonal

species reactivity

rat, human, bovine

manufacturer/tradename

Chemicon®

technique(s)

immunohistochemistry: suitable

isotype

IgM

NCBI accession no.

NM_006086.2

UniProt accession no.

Q13509

shipped in

dry ice

target post-translational modification

unmodified

Quality Level

100

Gene Information

human ... TTLL8(164714)

Application

Anti-Tubulin Antibody, β, clone 5H1 is an antibody against Tubulin for use in IH.
Immunohistochemistry at 1:300-1:1,000

Optimal working dilutions must be determined by end user.

IMMUNOHISTOCHEMISTRY PROTOCOL FOR MAB380

This antibody has been used successfully on 30 μm, free floating, 4% paraformaldehyde fixed rat brain tissue. All steps are performed under constant agitation. Suggested protocol follows.

1) 3 x 10 minute washes in TBS (with or without 0.25% Triton).

2) Incubate for 30 minutes in TBS with 3% serum (same as host from secondary antibody).

3) Incubate primary antibody diluted appropriately in TBS with 1% serum (same as host from secondary antibody) (with or without 0.25% Triton) for 2 hours at room temperature followed by 16 hours at 4°C.

4) 3 x 10 minute washes in TBS.

5) Incubate with secondary antibody diluted appropriately in TBS with1% serum (same as host from secondary antibody).

6) 3 x 10 minute washes in TBS.

7) ABC Elite (1:200 Vector Labs) in TBS.

8) 2 x 10 minute washes in TBS.

9) 1 x 10 minute wash in phosphate buffer (no saline).

10) DAB reaction with 0.06% NiCl added for intensification.

11) 2 x 10 minute washes in PBS.

12) 1 x 10 minute wash in phosphate buffer (no saline).
Research Category
Cell Structure
Research Sub Category
Cytoskeleton

Biochem/physiol Actions

Beta tubulin

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Immunogen

Rat tubulin.

Physical form

Ascites fluid containing sodium azide. Liquid

Preparation Note

Maintain at -20°C in undiluted aliquots for up to twelve months. Avoid repeated freeze/thaw cycles.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

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保管分類

12 - Non Combustible Liquids

wgk

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable

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Human family with sequence similarity 60 member A (FAM60A) protein: a new subunit of the Sin3 deacetylase complex.
Smith, KT; Sardiu, ME; Martin-Brown, SA; Seidel, C; Mushegian, A; Egidy, R; Florens et al.
Molecular and Cellular Proteomics null
Alexandre Fifre et al.
The Journal of biological chemistry, 281(1), 229-240 (2005-10-20)
A growing body of evidence supports the notion that soluble oligomeric forms of the amyloid beta-peptide (Abeta) may be the proximate effectors of neuronal injuries and death in the early stages of Alzheimer disease. However, the molecular mechanisms associated with
Central nervous system delivery of the antipsychotic olanzapine induces hepatic insulin resistance.
Martins, PJ; Haas, M; Obici, S
Diabetes null
Activation of platelet-activating factor receptor and pleiotropic effects on tyrosine phospho-EGFR/Src/FAK/paxillin in ovarian cancer.
Margarita Aponte, Wei Jiang, Montaha Lakkis, Ming-Jiang Li, Dale Edwards, Lina Albitar et al.
Cancer Research null
Kyle S McCommis et al.
Molecular metabolism, 5(8), 602-614 (2016-09-23)
Transport of pyruvate into the mitochondrial matrix by the Mitochondrial Pyruvate Carrier (MPC) is an important and rate-limiting step in its metabolism. In pancreatic β-cells, mitochondrial pyruvate metabolism is thought to be important for glucose sensing and glucose-stimulated insulin secretion.
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関連コンテンツ

Human Stem Cell Systems

As the focus of stem cell research undergoes a transition from animal to human models, researchers are in critical need of validated products to support the isolation, maintenance, differentiation, and characterization of human stem cells. While many reagents designed for rodent systems can be applied to human stem cell studies, they are not truly optimized for robust human stem cell culture or analysis. This is why human stem cell researchers have always trusted EMD Millipore, the first provider of commercially available human embryonic stem cells and human neural stem cell lines, to accelerate their research. All of our human stem cell systems are extensively tested in defined media culture, and differentiated progeny are comprehensively characterized with highly validated antibodies and detection reagents.

グローバルトレードアイテム番号

カタログ番号GTIN
MAB38004053252360855

ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.

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