MAB388
Anti-Myelin Proteolipid Protein Antibody, CT, clone PLPC1
clone PLPC1, Chemicon®, from mouse
別名:
PLP
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この商品について
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
PLPC1, monoclonal
Application:
IHC (p), WB
Technique(s):
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable, western blot: suitable
Citations:
24
Uniprot accession no.:
biological source
mouse
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
PLPC1, monoclonal
species reactivity (predicted by homology)
mammals
manufacturer/tradename
Chemicon®
technique(s)
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable, western blot: suitable
isotype
IgG2a
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Quality Level
Gene Information
human ... PLP1(5354)
Analysis Note
Control
Brain
Brain
Application
Detect Myelin Proteolipid Protein using this Anti-Myelin Proteolipid Protein Antibody, C-terminus, clone PLPC1 validated for use in IH, IH(P) & WB.
Research Category
Neuroscience
Neuroscience
Research Sub Category
Neuronal & Glial Markers
Neurochemistry & Neurotrophins
Neuronal & Glial Markers
Neurochemistry & Neurotrophins
Western Blotting: detects a 23-25kDa protein and DM20 a 20kDa protein; PLP can aggregate causing HMW bands to sometimes appear in certain preparations.
Immunocytochemistry: 4% PFA fixed cells, 5-10 minutes room temperature. Use 0.1% triton in block only to allow antibody access to C-terminal epitope.
Immunohistology on fresh frozen sections or 4% PFA fixed, frozen tissues. Epitope is internal, use 0.1-0.2% triton X-100 in the blocking step for permeabilization.
Immunohistochemistry in paraffin: works best with lightly fixed tissues, or Bouin′s fixed material, although there are reports of traditional formalin fixation also working with antigen recovery. {Mathis, et al 2003}.
Optimal working dilutions must be determined by end user.
Immunocytochemistry: 4% PFA fixed cells, 5-10 minutes room temperature. Use 0.1% triton in block only to allow antibody access to C-terminal epitope.
Immunohistology on fresh frozen sections or 4% PFA fixed, frozen tissues. Epitope is internal, use 0.1-0.2% triton X-100 in the blocking step for permeabilization.
Immunohistochemistry in paraffin: works best with lightly fixed tissues, or Bouin′s fixed material, although there are reports of traditional formalin fixation also working with antigen recovery. {Mathis, et al 2003}.
Optimal working dilutions must be determined by end user.
Biochem/physiol Actions
Reacts with myelin proteolipid protein in many mammalian species. Shows cross-reactivity to DM20.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description
23-25 kDa
Immunogen
Epitope: C-terminus
Myelin proteolipid protein, synthetic peptide CGRGTKF corresponding to the C-terminal peptide.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Replaces: MAB388
Physical form
Format: Purified
Protein A purified
Purified mouse immunoglobulin in PBS, pH 7.2 containing 0.09% sodium azide.
Preparation Note
Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
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保管分類
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
MAB388-100UG:
jan
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
Guidance of glial precursor cell migration by secreted cues in the developing optic nerve.
Sugimoto, Y, et al.
Development, 128, 3321-3330 (2001)
D W Bayless et al.
Neuroscience, 300, 286-296 (2015-05-24)
Impulsive actions and decisions often lead to undesirable outcomes. Lesion and neuroimaging studies have revealed that the orbital frontal cortex (OFC) and dorsal striatum (dSTR) play key roles in inhibitory control. It has been proposed that greater OFC input into
Raquel Bello-Morales et al.
PloS one, 9(2), e89141-e89141 (2014-02-20)
Herpes simplex type 1 (HSV-1) is a neurotropic virus that infects many types of cells. Previous studies have demonstrated that oligodendrocytic cells are highly susceptible to HSV-1 infection. Here we analysed HSV-1 infection of a human oligodendrocytic cell line, HOG
C M Cheng et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 18(15), 5673-5681 (1998-07-22)
To elucidate the role of insulin-like growth factor 1 (IGF1) in the normal development of brain myelination, we used behavioral, biochemical, and histological analyses to compare the myelination of brains from Igf1(-/-) and wild-type (WT) littermate mice. The studies were
Shih-Min Cheng et al.
Developmental neurobiology, 67(14), 1957-1974 (2007-10-06)
In the barn owl, maps of interaural time difference (ITD) are created in the nucleus laminaris (NL) by interdigitating axons that act as delay lines. Adult delay line axons are myelinated, and this myelination is timely, coinciding with the attainment
資料
Derivation and characterization of functional human neural stem cell derived oligodendrocyte progenitor cells (OPCs) that efficiently myelinate primary neurons in culture.
グローバルトレードアイテム番号
| カタログ番号 | GTIN |
|---|---|
| MAB388-100UG | 04053252360152 |
ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.
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