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Merck

MSHMAG-21K

Millipore

MILLIPLEX® Multi-Species Hormone Magnetic Bead Panel

The Multi-Species Hormone Panel, using the Luminex xMAP technology, enables the simultaneous analysis of 6 steroid/thyroid biomarkers in human serum, plasma and cell culture samples.

別名:

luminex multiple species thyroid reproductive stress endocrine metabolic hormones multiplex assay, millipore multiple species metabolism hormones multiplex kit, multi species hormone immunoassay panel

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この商品について

UNSPSCコード:
12161503
eCl@ss:
32161000
NACRES:
NA.47
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品質水準

交差性

human, canine

メーカー/製品名

Milliplex®

assay range

accuracy: 105%
(Canine Samples: T3)

accuracy: 80%
(Canine Samples: Estradiol)

accuracy: 85%
(Human Samples: Progesterone)

accuracy: 87%
(Canine Samples: T4)

sensitivity: 0.01 ng/mL
(MinDC+2SD; Estradiol)

sensitivity: 0.12 ng/mL
(MinDC+2SD; T3)

sensitivity: 0.25 ng/mL
(MinDC+2SD; Progesterone)

sensitivity: 0.33 ng/mL
(MinDC+2SD; Cortisol)

sensitivity: 0.51 ng/mL
(MinDC+2SD; T4)

standard curve range: 0.014-10 ng/mL
(Estradiol)

standard curve range: 0.07-50 ng/mL
(T3)

standard curve range: 0.14-100 ng/mL
(Progesterone)

standard curve range: 0.27-200 ng/mL
(Cortisol)

standard curve range: 0.34-250 ng/mL
(T4)

inter-assay cv: <10%
intra-assay cv: <10%
(Cortisol)

inter-assay cv: <15%
intra-assay cv: <10%
(Estradiol)

inter-assay cv: <15%
intra-assay cv: <10%
(Progesterone)

inter-assay cv: <15%
intra-assay cv: <10%
(T3)

inter-assay cv: <15%
intra-assay cv: <10%
(T4)

テクニック

multiplexing: suitable

検出方法

fluorometric (Luminex xMAP)

輸送温度

ambient

詳細

Steroid and thyroid hormones are small molecule endocrine hormones secreted from adrenal glands (cortisol), ovaries (estradiol and progesterone), testes (testosterone), and the thyroid (T3 and T4). These hormones play key roles in the regulation of energy balance, mental and physical development, sex organ development, reproduction, and pregnancy. These hormones play a role in the patho-physiology of many diseases such as thyroid-related diseases (Grave’s disease and hyper/hypothyroidism), steroid-related diseases (Cushing’s syndrome) and stress related diseases. Endocrine disrupting chemincals (EDC) can impact normal functioning of steroid and thyroid hormones.



The MILLIPLEX® Multi-Species Hormone Panel is a 6-plex kit to be used for the simultaneous quantification of any or all of the following analytes in serum, plasma and cell/tissue culture samples: Cortisol, Estradiol, Testosterone, Progesterone, T3 and T4. This multi-species kit may be used to detect these analytes in human, dog, rat, mouse, cynomolgous monkey, rhesus monkey, horse, rabbit, cat, guinea pig, pig and hamster. The kit has been verified with human and canine samples. The amount of cross-reactivity to other species has been confirmed, but not tested to the same level of verification as was used for human and canine. All analytes in this kit are run in a competitive assay format. This kit uses a 96-well format, contains: cocktail of lyophilized standard′s, 4 internal quality controls- 2 each for Testosterone and 2 for other 5 analytes and can measure up to 38 samples in duplicates.



The Luminex® xMAP® platform uses a magnetic bead immunoassay format for ideal speed and sensitivity to quantitate multiple analytes simultaneously, dramatically improving productivity while conserving valuable sample volume.



Panel Type: Endocrine

アプリケーション

  • Analytes: Cortisol, Estradiol, Progesterone, T3, T4, Testosterone.
  • NOTE: This multi-species kit may be used to detect these analytes in human, dog, rat, mouse, cynomolgus monkey, rhesus monkey, horse, rabbit, cat, guinea pig, pig and hamster. However, the kit was validated with human and canine samples only and the amount of cross-reactivity to other species tested, has not been determined.
  • NOTE: This assay requires an extraction step for serum and plasma samples (instructions are provided in the kit protocol).
  • NOTE: This is a competitive immunoassay
  • Recommended Sample Type: Serum, plasma, or tissue/cell culture supernatants or lysates from multiple species
  • Recommended Sample Dilution: 25 μL per well of undiluted extracted serum or plasma. Tissue/cell culture samples may be run neat or diluted in an appropriate control media.
  • Assay Run Time: Overnight (16-20 hours) at 2-8°C.
  • Research Category: Metabolism
  • Research Category: Endocrine

生物化学的/生理学的作用

Approximately 10% cross-reactivity to Cortisol is observed in the Progesterone assay and approximately 5% cross-reactivity to T3 is observed in the T4 assay. No other significant cross-reactivity is observed within the panel or to other similar analytes.

This multi-species kit may be used to detect these 6 analytes in human, dog, rat, mouse, cynomolgous monkey, rhesus monkey, horse, rabbit, cat, guinea pig, pig and hamster. However, the kit was fully validated with human and canine samples only and the amount of cross-reactivity to other species tested has not been determined.
Cross Reactivty
Approximately 10% cross-reactivity to Cortisol is observed in the Progesterone assay and approximately 5% cross-reactivity to T3 is observed in the T4 assay. No other significant cross-reactivity is observed within the panel or to other similar analytes.

特徴および利点

Design your multiplex kit by choosing available analytes within this panel.

包装

Everything you need in a single kit.
96 well plate

調製ノート

Recommended storage for kit components is 2 - 8°C.

その他情報

Please contact Technical Service for linearity of dilution.
Replaces: STTHMAG-21K

法的情報

Luminex is a registered trademark of Luminex Corp
MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany
xMAP is a registered trademark of Luminex Corp

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

ピクトグラム

Health hazardCorrosionExclamation markEnvironment

シグナルワード

Danger

危険有害性の分類

Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Skin Sens. 1 - STOT RE 2

ターゲットの組織

Respiratory Tract

保管分類コード

10 - Combustible liquids

WGK

WGK 3


適用法令

試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。

毒物及び劇物取締法

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PRTR

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消防法

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労働安全衛生法名称等を表示すべき危険物及び有害物

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労働安全衛生法名称等を通知すべき危険物及び有害物

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カルタヘナ法

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Jan Code

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試験成績書(COA)

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関連コンテンツ

新しいxMAP® Sheath Fluid PLUSの組成には、薬剤耐性菌の発生を抑制するために3種類の防腐剤が含まれています。MILLIPLEX®マルチプレックスアッセイにおける、従来のxMAP® Sheath Fluidと新しいxMAP® Sheath Fluid PLUSの性能比較をご覧ください。

Multiplex immunoassays, such as MILLIPLEX® multiplex metabolic assays, are critical in metabolic syndrome research because they provide a full picture of the different conditions related to it, like diabetes and obesity, as well as save time and sample volume.

実験動物、コンパニオンアニマル、農業用動物の研究におけるバイオマーカー検出マルチプレックス・イムノアッセイの利点と、獣医学および動物衛生研究のためのMILLIPLEX®マルチプレックスアッセイについて説明します。

すべての関連コンテンツを見る
Gabriela Leite et al.
Menopause (New York, N.Y.), 29(3), 264-275 (2022-02-26)
Hormone therapy (HT) is used to treat menopause-related conditions and symptoms. The small intestine plays key roles in metabolic and endocrine function, but the effects of HT on the small intestinal microbiome are unknown. Here, we characterize duodenal microbiome differences
Christian K Garcia et al.
PloS one, 17(10), e0275715-e0275715 (2022-10-14)
The capability of male mice to exercise in hot environments without succumbing to exertional heat stroke (EHS) is markedly blunted compared to females. Epidemiological evidence in humans and other mammals also suggests some degree of greater vulnerability to heat stroke