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Merck

PC130

Anti-Rad51 (Ab-1) Rabbit pAb

liquid, Calbiochem®

別名:

Anti-Rad51, Rabbit Anti-Rad51, Rad51 Detection Antibody

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この商品について

NACRES:
NA.41
UNSPSC Code:
12352203
Clone:
polyclonal
Species reactivity:
mouse, rat, human
Application:
—
Citations:
74

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biological source

rabbit

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

liquid

contains

≤0.1% sodium azide as preservative

species reactivity

mouse, rat, human

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze

dilution

(Immunoblotting (1:2500)
Immunoprecipitation (40 µL/500µg total protein from lysate)
Immunocytochemistry (1:500))

isotype

IgG

shipped in

wet ice

Quality Level

100

Gene Information

human ... RAD51(5888)

storage temp.

2-8°C

target post-translational modification

unmodified

General description

Affinity purified rabbit polyclonal antibody. Recognizes the ~43 kDa Rad51 protein.
Recognizes the ~43 kDa Rad51 protein in ovarian tissue.
The bacterial RecA and the yeast RAD51 proteins are involved in homologous recombination and DNA repair. The human RAD51 protein is the homologue of these two proteins. While the modular structure of these proteins is conserved and the homology is very strong with the bacterial protein there are functional differences seen with RAD51 including an ATP hydrolysis rate 1/10 that seen with RecA. Although RAD51 is a nuclear protein and is largely absent from the cytoplasm and nucleoli, the protein lacks a nuclear localization signal and requires other proteins in a complex to be translocated into the nucleus. Treatment of cells with DNA synthesis inducing agents will cause an increase in the levels of RAD51 protein which then undergo a shift in distribution becoming focal in nature. This focal distribution may be blocked by inhibitors of transcription. RAD51 can be shown to interact directly with the chromosomes and during the process of meiosis, its distribution of RAD51 undergoes dynamic change. RAD51 has been shown to interact with a wide variety of different proteins that include the wild-type form of p53, BRCA2, and RAD52, which specifically modulates its activity.
This Anti-Rad51 (Ab-1) Rabbit pAb is validated for use in Immunoblotting, Immunoprecipitation, Immunocytochemistry for the detection of Rad51 (Ab-1).

Immunogen

Human
recombinant, human Rad51

Application




Immunoblotting (1:2500)
Immunoprecipitation (40 l/500g total protein from lysate)
Immunocytochemistry (1:500)

Physical form

In 150 mM NaCl, 100 mM Tris-Glycine, pH 7.4

Preparation Note

Following initial thaw, aliquot and freeze (-20°C).

Analysis Note

Positive Control
A431 cells, HeLa cells

Other Notes

Brendel, V., et al. 1997. J. Mol. Evol.44, 528.
Boulikas, T., et al. 1997. Anticancer Res.17, 843.
Gupta, R.C., et al. 1997. Proc. Natl. Acad. Sci. USA94, 463.
Mizuta, R., et al. 1997. Proc. Natl. Acad. Sci. USA 94, 6927.
Sharan, S.K., et al. 1997. Nature386, 304.
Li, M.J., et al. 1996. Proc. Natl. Acad. Sci. USA93, 10222.
Shen, Z., et al. 1996. J. Biol. Chem.271, 148.
Sturzbecher, H.W., et al. 1996. EMBO J. 15, 1992.
Tashiro, S., et al. 1996. Oncogene12, 2165.
Ashley, T., et al. 1995. Chromosoma104, 19.
Haaf, T., et al. 1995. Proc. Natl. Acad. Sci. USA92, 2298.
Due to a high degree of sequence identity, this antibody is also expected to cross-react with bovine, equine, and canine RAD51. Antibody should be titrated for individual systems.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

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保管分類

12 - Non Combustible Liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

適用法令

試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。

PC130-100UL:

jan

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文書ライブラリにアクセスする

Wataru Sakai et al.
Nature, 451(7182), 1116-1120 (2008-02-12)
Ovarian carcinomas with mutations in the tumour suppressor BRCA2 are particularly sensitive to platinum compounds. However, such carcinomas ultimately develop cisplatin resistance. The mechanism of that resistance is largely unknown. Here we show that acquired resistance to cisplatin can be
Yue Sun et al.
Cell death & disease, 11(4), 292-292 (2020-04-29)
Genome instability is the fundamental hallmark of malignant tumors. Tumor suppressors often play a role in maintaining genome stability. Our previous genetic screen identified inositol polyphosphate 4-phosphatase type B (INPP4B), primarily hydrolyzing phosphatidylinositol 3, 4-disphosphate, is a potential tumor suppressor
Yasuhiro Murakawa et al.
Cancer research, 67(18), 8536-8543 (2007-09-19)
Proteasome inhibitors are novel antitumor agents against multiple myeloma and other malignancies. Despite the increasing clinical application, the molecular basis of their antitumor effect has been poorly understood due to the involvement of the ubiquitin-proteasome pathway in multiple cellular metabolisms.
Eddy S Yang et al.
The Journal of clinical investigation, 119(5), 1124-1135 (2009-05-09)
Long-term neurological deficiencies resulting from hippocampal cytotoxicity induced by cranial irradiation (IR) present a challenge in the treatment of primary and metastatic brain cancers, especially in children. Previously, we showed that lithium protected hippocampal neurons from IR-induced apoptosis and improved
Laura A Bannister et al.
PLoS biology, 5(5), e105-e105 (2007-04-12)
DMC1 is a meiosis-specific homolog of bacterial RecA and eukaryotic RAD51 that can catalyze homologous DNA strand invasion and D-loop formation in vitro. DMC1-deficient mice and yeast are sterile due to defective meiotic recombination and chromosome synapsis. The authors identified
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グローバルトレードアイテム番号

カタログ番号GTIN
PC130-100ULCN07790788053949

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