PC386
PhosphoDetect Anti-p53 (pSer¹⁵) (Ab-3) Rabbit pAb
liquid, Calbiochem®
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この商品について
NACRES:
NA.41
UNSPSC Code:
12352203
Clone:
polyclonal
Species reactivity:
rat, mouse, human
Application:
—
Citations:
5
biological source
rabbit
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
form
liquid
does not contain
preservative
species reactivity
rat, mouse, human
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
dilution
(Immunoblotting (1:1000)
Immunoprecipitation (1:200))
isotype
IgG
shipped in
wet ice
storage temp.
−20°C
target post-translational modification
phosphorylation (pSer15)
Quality Level
Gene Information
human ... TP53(7157)
Analysis Note
Positive Control
PC-12, HCT116, or HeLa cells treated with DNA damaging agents
PC-12, HCT116, or HeLa cells treated with DNA damaging agents
Application
Immunoblotting (1:1000)
Immunoprecipitation (1:200)
Disclaimer
Toxicity: Standard Handling (A)
General description
Immunoaffinity purified rabbit polyclonal antibody. Recognizes the ~53 kDa p53 protein phosphorylated on Ser15.
Recognizes the ~53 kDa p53 protein phosphorylated at Ser15 in PC12 or HeLa cells treated with DNA damaging agents. Does not recognize unphosphorylated p53.
This PhosphoDetect Anti-p53 (pSer¹⁵) (Ab-3) Rabbit pAb is validated for use in Immunoblotting , Immunoprecipitation for the detection of p53 (pSer¹⁵) (Ab-3).
Immunogen
Human
a synthetic phosphopeptide corresponding to amino acids surrounding the Ser¹⁵ phosphorylation site of human p53, conjugated to KLH
Other Notes
Does not recognize non-phosphorylated p53. Antibody should be titrated for optimal results in individual systems.
Tibbetts, R.S., et al. 1999. Genes Dev.13, 152.
Levine, A.J. 1997. Cell88, 323.
Milczarek, G.J., et al. 1997. Life Sci.60, 1.
Shieh, S.Y., et al. 1997. Cell91, 325.
Meek, D.W. 1994. Semin Cancer Biol.5, 203.
Levine, A.J. 1997. Cell88, 323.
Milczarek, G.J., et al. 1997. Life Sci.60, 1.
Shieh, S.Y., et al. 1997. Cell91, 325.
Meek, D.W. 1994. Semin Cancer Biol.5, 203.
Physical form
In 150 mM NaCl, 10 mM sodium HEPES, 100 µg/ml BSA, 50% glycerol, pH 7.5.
Preparation Note
Following initial thaw, aliquot and freeze (-20°C). Avoid freeze/thaw cycles.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
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保管分類
10 - Combustible liquids
wgk
WGK 2
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
PC386-50UL: + PC386-ML:
jan
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
Timothy R Schwartz et al.
BMC molecular biology, 8, 7-7 (2007-01-30)
The repair of single base mutations in mammalian genes can be directed by single-stranded oligonucleotides in a process known as targeted gene repair. The mechanism of this reaction is currently being elucidated but likely involves a pairing step in which
Rene Rodriguez et al.
Neoplasia (New York, N.Y.), 11(4), 397-407 (2009-03-25)
There is growing evidence about the role of mesenchymal stem cells (MSCs) as cancer stem cells in many sarcomas. Nevertheless, little is still known about the cellular and molecular mechanisms underlying MSCs transformation. We aimed at investigating the role of
Jasmine R Elison et al.
Archives of ophthalmology (Chicago, Ill. : 1960), 124(9), 1269-1275 (2006-09-13)
To determine the efficacy of inducing p53-mediated cell death in retinoblastoma cells by Nutlin 3A, a small molecule HDM2 inhibitor. Retinoblastoma cell lines WERI-RB-1 and Y79 were treated with Nutlin 3A. Cell viability assays, Western blot analyses, confocal microscopy, and
Awad Shamma et al.
Molecular and cellular biology, 33(16), 3113-3124 (2013-06-12)
The retinoblastoma tumor suppressor gene (RB) product has been implicated in epigenetic control of gene expression owing to its ability to physically bind to many chromatin modifiers. However, the biological and clinical significance of this activity was not well elucidated.
Stefano J Mandriota et al.
The Journal of biological chemistry, 285(17), 13092-13106 (2010-02-24)
Carriers of mutations in the cell cycle checkpoint protein kinase ataxia telangiectasia mutated (ATM), which represent 1-2% of the general population, have an increased risk of breast cancer. However, experimental evidence that ATM deficiency contributes to human breast carcinogenesis is
グローバルトレードアイテム番号
| カタログ番号 | GTIN |
|---|---|
| PC386-50ULCN | 04055977224559 |
ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.
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