Heregulin, Human, Recombinant, E. coli

Proliferation Studies

EC₅₀ ~20-40 ng/ml as determined by a cell proliferation assay using breast carcinoma cell line SK-BR-3 or in a serum-free cell proliferation assay using the human cell line MCF7.

Toxicity: Standard Handling (A)

Recombinant, human heregulin expressed in E. coli. Human recombinant heregulin whose DNA sequence encoding the EGF domain of heregulin α (amino acids 177-241) expressed in and purified from E. coli. The expressed protein consists of 65 amino acids with a predicted molecular weight of approximately 7 kDa.

It is recommended that the protein be titrated for optimal results in individual systems.

Riese, D.J., et al. 1996. J. Biol. Chem.271, 20047.
Holmes, W.E., et al. 1992. Science256, 1205.
Karey, K.P., et al. 1988. Cancer Res.48, 4083.

Lyophilized from PBS, 50 µg BSA/µg cytokine.

Store lyophilized protein dessicated at -20°C. Lyophilized product should be reconstituted in sterile PBS containing at least 0.1% human serum albumin or bovine serum albumin to prepare a stock solution of no less than 50 µg/ml. Following initial thaw, aliquot and freeze (-20°C).

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