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Merck

SCM075

ROCK阻害薬（Y-27632）

The ROCK Inhibitor (Y-27632) is available in a 5 mg format & has been optimized & validated for cell culture & Neuroscience applications.

別名:

Y-27632

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この商品について

UNSPSCコード:
12352207
eCl@ss:
32160801
NACRES:
NA.75

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品質水準

100

フォーム

liquid

分子量

320.26 g/mol

テクニック

cell culture | mammalian: suitable

輸送温度

dry ice

関連するカテゴリー

詳細

ROCK阻害薬（Y-27632）は、p160Rock（Ki=140 nM）およびRock-IIなどのRho関連プロテインキナーゼ（ROCK）の選択的な、ATP競合的阻害薬です。また、アゴニストによるミオシンリン酸化のCa2+感作と平滑筋の収縮に対する強力な阻害薬としても作用します。10uM Y-27632による1時間の処理は、解離した培養ヒト胚性幹細胞（hESC）のアポトーシスを阻害し、その多能性に影響を及ぼすことなくhESCの生存とクローニング効率を高めます1。最近では、Y-27632や消化酵素の調製混合物であるAccutase（カタログ番号：SCR005）が、従来の緩慢凍結および急速融解法を用いて単一hESCの融解後の生存率を高めることが報告されています。

調製ノート

溶解前：遮光して-20℃で2年間保存できます。溶解後：遮光して-20℃で1年間保存できます。

保管分類コード

11 - Combustible Solids

WGK

WGK 3

引火点(°F)

Not applicable

引火点(℃)

Not applicable

適用法令

試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。

Jan Code

SCM075:

試験成績書（COA）

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Katarzyna Pańczyk et al.
MedChemComm, 9(11), 1933-1948 (2018-12-21)
A series of 17 new phenoxyacetamides has been prepared via multistep chemical synthesis as a continuation of the research carried out by our group on di- and tri-substituted phenoxyalkyl and phenoxyacetyl derivatives of amines. The obtained compounds vary in an
Maike Frye et al.
eLife, 9 (2020-09-09)
Endothelial integrity is vital for homeostasis and adjusted to tissue demands. Although fluid uptake by lymphatic capillaries is a critical attribute of the lymphatic vasculature, the barrier function of collecting lymphatic vessels is also important by ensuring efficient fluid drainage
Xin Geng et al.
JCI insight, 5(14) (2020-06-17)
During the growth of lymphatic vessels (lymphangiogenesis), lymphatic endothelial cells (LECs) at the growing front sprout by forming filopodia. Those tip cells are not exposed to circulating lymph, as they are not lumenized. In contrast, LECs that trail the growing
Anushree C Gulvady et al.
Molecular biology of the cell, 30(11), 1298-1313 (2019-03-21)
Fibroblasts transformed by the proto-oncogene Src form individual invadopodia that can spontaneously self-organize into large matrix-degrading superstructures called rosettes. However, the mechanisms by which the invadopodia can spatiotemporally reorganize their architecture is not well understood. Here, we show that Hic-5
Xin Lin et al.
EBioMedicine, 69, 103463-103463 (2021-07-06)
Family with Sequence Similarity 13, Member A (FAM13A) gene has been consistently associated with COPD by Genome-wide association studies (GWAS). Our previous study demonstrated that FAM13A was mainly expressed in the lung epithelial progenitors including Club cells and alveolar type
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資料

Using Human iPS Cell-derived Colon Organoids for Cytotoxicity Screening of Drug Compounds

Human epithelial intestinal colonic organoids can be used as an alternative to Caco-2 drug permeability assays for drug screening and compound toxicity testing.

Organoid Culture Frequently Asked Questions

Organoid culture FAQs. Learn how to culture and analyze organoids following established 3D cell culture protocols. Tips and tricks.

In Vitro Differentiation of Human iPS Cells into Airway Epithelial Lung Organoids for Respiratory Disease Research Applications

Lung organoids are valuable 3D models for human lung development and respiratory diseases. The 3dGRO™ differentiation protocol generates organoids from human iPSCs in 4 steps.

A Serum-Free and Feeder-Free Defined Medium That Enables Weekend-Free Culture of Undifferentiated Human ES/iPS Cells

Skip weekend feedings. Defined serum-free and feeder-free expansion media for human pluripotent stem cells (ES and iPS cells). See publications and protocols.

プロトコル

Forskolin-Induced Swelling Assays using Millicell® Microwell Plates

Learn how to cultivate similar-sized iPSC-derived colon organoids using Millicell® Microwell plates and perform a forskolin-induced swelling assay.

In Vitro Differentiation of Human iPS Cells Into Colon Organoids in Serum-Free Cell Culture Conditions

Highly characterized cryopreserved human colonic organoids and a step-by-step organoid culture protocol for epithelial intestinal organoid differentiation from iPS cells.

Forskolin-Induced Swelling Assays

A rapid in vitro assay for CFTR function, the forskolin-induced swelling protocol uses human colon organoids, which can be derived from cystic fibrosis patient tissue.

Induced Pluripotent Stem Cell Culture Protocols

Step-by-step stem cell culture protocols for human induced pluripotent stem cells (iPSCs) including ips cell thawing, expanding, freezing and characterizing.

関連コンテンツ

Human Stem Cell Systems

As the focus of stem cell research undergoes a transition from animal to human models, researchers are in critical need of validated products to support the isolation, maintenance, differentiation, and characterization of human stem cells. While many reagents designed for rodent systems can be applied to human stem cell studies, they are not truly optimized for robust human stem cell culture or analysis. This is why human stem cell researchers have always trusted EMD Millipore, the first provider of commercially available human embryonic stem cells and human neural stem cell lines, to accelerate their research. All of our human stem cell systems are extensively tested in defined media culture, and differentiated progeny are comprehensively characterized with highly validated antibodies and detection reagents.

Culturing Colon Organoid Monolayers using Millicell® Hanging Inserts

Monitor barrier formation using colon PDOs, iPSC-derived colon organoids, Millicell® cell culture inserts, and the Millicell® ERS. 3.0.

グローバルトレードアイテム番号

カタログ番号GTIN
SCM07504053252374654

ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.

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