製品名
TUNEL AP, sufficient for 70 tests, solution, pkg of 3.5 mL
form
solution
usage
sufficient for 70 tests
packaging
pkg of 3.5 mL
manufacturer/tradename
Roche
shipped in
wet ice
storage temp.
2-8°C
Application
TUNEL AP is an antibody that is used to convert fluorescence-based TUNEL assays into colorimetric assays suited for light microscopy. The conversion is performed by binding of an anti-fluorescein antibody to FITC-dUTP. The antibody is labeled with alkaline phosphatase (AP). The AP is visualized with a precipitating substrate, such as Fast Red or NBT/BCIP.
General description
TUNEL AP is an alkaline phosphatase-labeled antibody used for the in situ detection of apoptosis (programmed cell death) with the TUNEL reaction followed by transmission light microscopy.
The tailing reaction using TdT, also named ISEL (in situ end labeling) or TUNEL (TdT-mediated dUTP nick end labeling), has several advantages in comparison to the in situ nick translation (ISNT) using DNA polymerase:
- Label intensity of apoptotic cells is higher with TUNEL compared to ISNT, resulting in an increased sensitivity.
- Kinetics of nucleotide incorporation is very rapid with TUNEL compared to the ISNT.
- TUNEL preferentially labels apoptotic cells compared to necrotic cells.
Other Notes
Anti-fluorescein antibody, Fab fragment from sheep, conjugated with alkaline phosphatase (AP). Ready-to-use solution.
For life science research only. Not for use in diagnostic procedures.
signalword
Warning
hcodes
Hazard Classifications
Skin Sens. 1
保管分類
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
No data available
flash_point_c
No data available
Simultaneous determination of cell surface antigens and apoptosis.
R Sgonc et al.
Trends in genetics : TIG, 10(2), 41-42 (1994-02-01)
C D Bortner et al.
Trends in cell biology, 5(1), 21-26 (1995-01-01)
The formation of distinct DNA fragments of oligonucleosomal size (180-200 bp lengths) is a biochemical hallmark of apoptosis in many cells. Recent observations also suggest large DNA fragments and even single-strand cleavage events occur during cell death. These observations have
R Gold et al.
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 41(7), 1023-1030 (1993-07-01)
Since DNA fragmentation is a key feature of programmed cell death (PCD) and also occurs in certain stages of necrosis, we have adapted the methodology of in situ nick-translation (ISNT) to detect DNA fragmentation on a single-cell level. We first
W Gorczyca et al.
Cytometry, 15(2), 169-175 (1994-02-01)
The predominant mode of either spontaneous or drug-induced death of cells in tumors is apoptosis. A flow cytometric method was developed in our laboratory to identify apoptotic cells, based on labeling DNA strand breaks, which appear as a result of
J P Heiserman et al.
Cell stress & chaperones, 20(3), 527-535 (2015-02-27)
Extracellular (ex) HSP60 is increasingly recognized as an agent of cell injury. Previously, we reported that low endotoxin exHSP60 causes cardiac myocyte apoptosis. Our findings supported a role for Toll-like receptor (TLR) 4 in HSP60 mediated apoptosis. To further investigate
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