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この商品について
実験式(ヒル表記法):
C9H18O5S
CAS番号:
分子量:
238.30
UNSPSC Code:
12352201
NACRES:
NA.31
PubChem Substance ID:
EC Number:
206-703-0
Beilstein/REAXYS Number:
4631
MDL number:
製品名
イソプロピル β-D-1-チオガラクトピラノシド, ≥99% (TLC)
InChI key
BPHPUYQFMNQIOC-NXRLNHOXSA-N
InChI
1S/C9H18O5S/c1-4(2)15-9-8(13)7(12)6(11)5(3-10)14-9/h4-13H,3H2,1-2H3/t5-,6+,7+,8-,9+/m1/s1
SMILES string
CC(C)S[C@@H]1OC(CO)[C@H](O)C(O)[C@H]1O
description
non-ionic
assay
≥99% (TLC)
form
powder
technique(s)
thin layer chromatography (TLC): suitable
impurities
10.0-22.0% Dioxane
color
white to off-white
mp
120 -122 °C ((248 - 252 °F ))
solubility
water: 50 mg/mL, clear, colorless
storage temp.
2-8°C
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Application
IPTGは、β-ガラクトシダーゼ活性の発現誘導が必要なクローニングの際に用いられます。大腸菌中のlacオペロンの発現を誘導させる組み換えコロニーのblue-white selectionの際に、X-GalまたはBluo-Gal とともに用います。β-ガラクトシダーゼをコードしているlacZ遺伝子を抑制しているlacIリプレッサーに結合してコンホメーションを変化させ、その抑制を妨げます。
Preparation Note
IPTGを水に溶解しフィルター滅菌してストック溶液 (0.1 M)を作成します。プレート中の最終濃度は0.2 mMです。
signalword
WarningDanger
Hazard Classifications
Carc. 1B - Eye Irrit. 2
supp_hazards
保管分類
6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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Pantothenate kinase (PanK) catalyzes the phosphorylation of pantothenate, the first committed and rate-limiting step toward coenzyme A (CoA) biosynthesis. In our earlier reports, we had established that the type I isoform encoded by the coaA gene is an essential pantothenate
ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.
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