RDD035
HEPES ナトリウム塩
≥99.5% (titration), free-flowing, Redi-Dri™
別名:
4-(2-ヒドロキシエチル)ピペラジン-1-エタンスルホン酸 ナトリウム塩, N-(2-ヒドロキシエチル)ピペラジン-N′-(2-エタンスルホン酸) ナトリウム塩
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この商品について
実験式（ヒル表記法）:
C8H17N2NaO4S
CAS番号:
分子量:
260.29
UNSPSC Code:
12161700
NACRES:
NA.25
MDL number:
製品名
HEPES ナトリウム塩, ≥99.5% (titration), free-flowing, Redi-Dri™
InChI
1S/C8H18N2O4S.Na/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14;/h11H,1-8H2,(H,12,13,14);/q;+1/p-1
SMILES string
[Na+].OCCN1CCN(CC1)CCS([O-])(=O)=O
InChI key
RDZTWEVXRGYCFV-UHFFFAOYSA-M
product line
Redi-Dri™
assay
≥99.5% (titration)
quality
free-flowing
useful pH range
6.8-8.2
pKa (25 °C)
7.5
application(s)
diagnostic assay manufacturing
Quality Level
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Physical form
凍結乾燥粉末、噴霧乾燥粉末、または結晶。
Application
HEPES sodium salt can be used:
- as a protein precipitation buffer
- in cell culture media
- as a binding buffer
Features and Benefits
- Redi-Dri™ eliminates clumping for hygroscopic chemicals
- powder remains free-flowing and easy to use
- Safer to handle and weigh out for standard use in lab
- Eliminates clumps, maintains product moisture, quality, and characteristics
General description
HEPES sodium salt is essential to prepare HEPES buffer that is commonly used for cell culture and protein applications. It is useful for maintaining homeostasis when working with cells outside of the incubator by balancing CO2. HEPES buffering capacity exceeds most buffers, with a pKa of 7.5, making it optimal for maintaining pH in biological systems. In solution, it is non-toxic, zwitterion with a physiological buffering range of pH 6.8 - 8.2. It has a low metal binding capacity and is safe to use in research for metal-binding proteins.
Other Notes
For additional information on our range of Biochemicals, please complete this form.
Legal Information
Redi-Dri is a trademark of Sigma-Aldrich Co. LLC
保管分類
13 - Non Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
Nishant Mehta et al.
Cell reports, 28(10), 2509-2516 (2019-09-05)
V-domain immunoglobulin (Ig) suppressor of T cell activation (VISTA) is an immune checkpoint protein that inhibits the T cell response against cancer. Similar to PD-1 and CTLA-4, a blockade of VISTA promotes tumor clearance by the immune system. Here, we report a
Åsa Birna Birgisdottir et al.
Autophagy, 15(8), 1333-1355 (2019-02-16)
Autophagosome formation depends on a carefully orchestrated interplay between membrane-associated protein complexes. Initiation of macroautophagy/autophagy is mediated by the ULK1 (unc-51 like autophagy activating kinase 1) protein kinase complex and the autophagy-specific class III phosphatidylinositol 3-kinase complex I (PtdIns3K-C1). The
Peng Yang
Macromolecular bioscience, 12(8), 1053-1059 (2012-06-19)
When lysozyme is dissolved in a neutral HEPES buffer solution (pH = 7.4) with 0.001-0.050 M TCEP added, a fast phase transition process occurs and the resulting novel fiber-like hierarchical supramolecular assemblies made by primary spherical-particle aggregation can function as a "superglue"
Hubert Gojzewski et al.
Scanning, 34(3), 159-169 (2011-09-29)
In this study, we deposited isolated magnetosomes from magnetotactic bacteria Magnetospirillum strain AMB-1 onto solid surfaces using spin coating (SC) and drop coating (DC) techniques. Four imaging techniques have been used to visualize the sample structure: scanning and transmission electron
Anca G Delgado et al.
Microbial cell factories, 11, 128-128 (2012-09-15)
Buffering to achieve pH control is crucial for successful trichloroethene (TCE) anaerobic bioremediation. Bicarbonate (HCO3-) is the natural buffer in groundwater and the buffer of choice in the laboratory and at contaminated sites undergoing biological treatment with organohalide respiring microorganisms.
資料
Redi-Dri™ prevents hygroscopic powders, such as inorganic salts, from absorbing moisture and forming clumps, leaving the salts free-flowing every time.
ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.
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