35845
DCFHDA
BioReagent, suitable for fluorescence, ≥95% (HPLC)
別名:
2′,7′-ジクロロフルオレセインジアセタート, ジアセチルジクロロフルオレセイン
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この商品について
実験式（ヒル表記法）:
C24H14Cl2O7
CAS番号:
分子量:
485.27
UNSPSC Code:
12352108
NACRES:
NA.32
PubChem Substance ID:
MDL number:
Beilstein/REAXYS Number:
68488
製品名
DCFHDA, BioReagent, suitable for fluorescence, ≥95% (HPLC)
InChI key
VQVUBYASAICPFU-UHFFFAOYSA-N
InChI
1S/C24H14Cl2O7/c1-11(27)30-21-9-19-15(7-17(21)25)24(14-6-4-3-5-13(14)23(29)33-24)16-8-18(26)22(31-12(2)28)10-20(16)32-19/h3-10H,1-2H3
SMILES string
CC(=O)Oc1cc2Oc3cc(OC(C)=O)c(Cl)cc3C4(OC(=O)c5ccccc45)c2cc1Cl
Quality Level
description
suitable as a fluorogenic substrate for esterases
product line
BioReagent
assay
≥95% (HPLC)
form
powder or crystals
mol wt
485.27 g/mol
color
faint red
white to faint brown
mp
232-234 °C (lit.)
solubility
DMSO: soluble
fluorescence
λex 504 nm; λem 524 nm in 0.1 M Tris pH 8.0 (esterase)
suitability
suitable for fluorescence
storage temp.
2-8°C
関連するカテゴリー
Application
2′,7′-ジクロロフルオレセインジアセテートは主に、活性酸素（ROS）中間体の検出と、細胞内の全体的な 酸化ストレス の定量に使用されます。
Dcfh-DAは、ナノ粒子処理細胞の酸化ストレスを測定するための定量アッセイに使用されます。
Dcfh-DAは、ナノ粒子処理細胞の酸化ストレスを測定するための定量アッセイに使用されます。
General description
2′,7′-ジクロロフルオレセインジアセテート（別名DCFHDAまたはDcfh-DA）は、高感度の細胞透過性酸化還元プローブです。親油性非蛍光Dcfh-DAは、受動拡散と後続の脱アセチル化によって細胞膜を容易に通過します。その脱アシル化産物が、酸化剤への感受性をもつ2′,7′-ジクロロフルオレセイン（DCHF）です。 DCHFはその後酸化され、測定対象となる高蛍光性のDCFが形成されます。
保管分類
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
dust mask type N95 (US), Eyeshields, Gloves
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