リパーゼ from Rhizopus oryzae

Research Area: Cell Signaling
Rhizopus oryzae lipase (ROL) is a protein synthesized in a precursor form that includes a presequence of 26 amino acids, followed by a prosequence of 97 amino acids, which is attached to the N-terminal of a mature sequence consisting of 269 amino acids.

Lipase from Rhizopus oryzae (ROL) comprises an oxyanion hole, four N-glycosylation sites, and an active site region. It possesses N-terminal presequence and prosequence.

産業分野においてリパーゼは、キラル化合物の分解、およびエステル交換によるバイオディーゼル生成に用いられます。

Lipase from Rhizopus oryzae has been used:

• to test its effect on 1,2-diolein synthesis and triolein ethanolysis
• for immobilization on graphene oxide support for biocatalysis studies
• to digest triglycerides (TAG) from Chlamydomonas reinhardtii and S. cerevisiae

トリグリセリド、ジグリセリド、モノグリセリドが加水分解されます(反応速度の高い順)。

リパーゼは、トリアシルグリセロールの加水分解を触媒し、グリセロールと遊離脂肪酸を生成します。

Rhizopus oryzae lipase (ROL) has been extensively researched for its regiospecificity in biodiesel production. Due to its remarkable characteristics, including 1,3-specificity, high enantioselectivity, and stability in organic solvents, ROL has garnered significant attention for applications in the energy, food, and pharmaceutical industries.

Lipase from Rhizopus oryzae (ROL) acts as a catalyst for the enzymatic biosynthesis of polyglycerol polyricinoleate through a reversal of hydrolysis. ROL is useful in the industrial production of structured lipids due to its 1,3-regiospecificity functionality.

1 Uは、pH 8.0、40°C、1分間で、1 μmolの酪酸を遊離させる酵素量に相当します(基質としてトリブチリン、製品番号91010)。上述の5,000ユニットは、pH 8.0、40°Cでトリオレイン(製品番号62314)を基質として用いた場合の約1 Uに相当します。

注:トリアセチンを基質として用いる場合、pHは7.4、インキュベ-ション時間は60分です。

Catalyst for the interesterification of oils and fats; For removal of interfering triglycerides in the electroimmunoassay of apolipoprotein B; Racemic epoxy ester resolution through enantioselective enzymatic hydrolysis.