パパイン from Carica papaya

パパインは食肉軟化剤として使用されます。また、初代細胞培養の調製において組織を細分化するためにも使用されます。

Papain from Carica papaya has been used:

• in the enzymatic dissociation of rat cortices to extract astrocytes for cell culture
• to study its effects on Alpaca seminal plasma viscosity and sperm function
• to study its effects on the yield and cryo-survival of Rhinoceros spermatozoa

パパインは、ペプチド結合を切断するシステインプロテアーゼであり、抗体のFc部分を切断することが知られています。

Papain plays an important role in many biological processes in many living organisms. It exhibits proteolytic activity against proteins, amino-acid esters and amide links, and short-chain peptides. Papain preferentially cleaves the peptide bonds of basic amino acids such as arginine, phenylalanine, and lysine. It has a wide range of applications in drug discovery, pharmaceutical, food, baking, and dairy industries.

1 unitは、pH 6.2、25°C、1分間で、1 μmolのN-ベンゾイル-L-アルギニンエチルエステル(BAEE、製品番号12880)を加水分解する酵素量に相当します。

Papain is a proteolytic enzyme and is naturally occurring in papaya (Carica papaya L.). It is obtained from the latex of cut unripe papaya. Papain belongs to the cysteine proteinase family. It is a globular, single-chain protein composed of four disulfide bridges and catalytically essential residues. Papain consists of an all α-domain and anti-parallel β-sheet domain.