A0856
抗ATG5 (N-末端) ウサギ宿主抗体
affinity isolated antibody, PBS solution
別名:
Anti-APG5, Anti-APG5L, Anti-ASP, Anti-ATG5 autophagy related 5 homolog (S. cerevisiae), Anti-Apoptosis-specific protein, Anti-Autophagy protein 5-like
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この商品について
Conjugate:
unconjugated
Clone:
polyclonal
Application:
IF, WB
Citations:
53
biological source
rabbit
Quality Level
conjugate
unconjugated
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
form
PBS solution
mol wt
antigen ~56 kDa (Atg5-Atg12 complex)
species reactivity
mouse, human, rat
packaging
antibody small pack of 25 μL
technique(s)
western blot: 0.5-1 μg/mL using whole extracts of human K562, rat NRK, and mouse 3T3 cells, immunofluorescence: suitable
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... ATG5(9474)
mouse ... Atg5(11793)
rat ... Atg5(365601)
Immunogen
ヒトATG5のアミノ酸2-15に相当する合成ペプチド(C末端システイン残基)のKLH結合体。この配列はラットおよびマウスにおいて同一です。
Application
Anti-ATG5 (N-terminal) antibody has been used:
- in immunoblotting
- in immunofluorescence
- in western blotting
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)
Western Blotting (1 paper)
Rabbit anti-ATG5 (N-terminal specific) was used for western blot analysis to detect the level of ATG5 in SK-N-MC cells after transduction with an adenovirus expressing a shRNA specific to ATG5.
Rabbit anti-ATG5 has been used for immunofluorescence and chemiluminescent western blot analyses.
Biochem/physiol Actions
Atg5 (Apg5) is a 32 kDa protein that is required for autophagy. Atg5 is covalently modified by Atg12, a ubiquitin-like modifier. The Atg12-Atg5-Atg16 complex thus formed, localizes to autophagosome precursors and regulates autophagosome formation. Studies have reported that Atg5 fragment that is formed by calpain cleavage has pro-apoptotic properties.
Rabbit anti-ATG5 (N-terminal) recognizes human, rat, and mouse Atg5-Atg12 complex.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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保管分類
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
A0856-25UL: + A0856-200UL: + A0856-BULK: + A0856-VAR: + IXO14080:
jan
The deubiquitinating enzyme USP36 controls selective autophagy activation by ubiquitinated proteins.
Emmanuel Taillebourg et al.
Autophagy, 8(5), 767-779 (2012-05-25)
Initially described as a nonspecific degradation process induced upon starvation, autophagy is now known also to be involved in the degradation of specific ubiquitinated substrates such as mitochondria, bacteria and aggregated proteins, ensuring crucial functions in cell physiology and immunity.
Shintaro Seto et al.
PloS one, 8(12), e86017-e86017 (2014-01-01)
Mycobacterium tuberculosis is an intracellular pathogen that can survive within phagocytic cells by inhibiting phagolysosome biogenesis. However, host cells can control the intracellular M. tuberculosis burden by the induction of autophagy. The mechanism of autophagosome formation to M. tuberculosis has
Kallikrein-8 inhibition attenuates Alzheimer's disease pathology in mice
Herring A, et al.
Alzheimers Dement., 12(12), 1273-1287 (2016)
Denitsa S Petkova et al.
Viruses, 9(5) (2017-05-23)
Autophagy is a potent cell autonomous defense mechanism that engages the lysosomal pathway to fight intracellular pathogens. Several autophagy receptors can recognize invading pathogens in order to target them towards autophagy for their degradation after the fusion of pathogen-containing autophagosomes
Autophagy adaptor protein p62/SQSTM1 and autophagy-related gene Atg5 mediate autophagosome formation in response to Mycobacterium tuberculosis infection in dendritic cells
Seto S, et al.
PLoS ONE, 8(12), e86017-e86017 (2013)
関連コンテンツ
ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.
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