製品名
モノクロナール抗β-アクチン マウス宿主抗体, clone AC-74, purified immunoglobulin, buffered aqueous solution
biological source
mouse
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
AC-74, monoclonal
form
buffered aqueous solution
mol wt
antigen 42 kDa
species reactivity
rabbit, sheep, cat, guinea pig, bovine, mouse, chicken, wide range, Hirudo medicinalis, canine, Drosophila, carp, pig, rat, human
should not react with
Dictyostelium discoideum
concentration
~2 mg/mL
technique(s)
immunocytochemistry: 10-40 μg/mL using human or chicken fibroblasts
immunohistochemistry: suitable
microarray: suitable
western blot: 0.5-1 μg/mL using total cell extracts of human or chicken fibroblasts
isotype
IgG2a
UniProt accession no.
application(s)
research pathology
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
Gene Information
human ... ACTB(60)
mouse ... Actb(11461)
rat ... Actb(81822)
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Other Notes
Physical form
Application
Biochem/physiol Actions
β-アクチンは、細胞増殖、細胞遊走、およびG-アクチンプールを調節します。ACTBにおける変異は、多面的な発達障害を引き起こします。
Disclaimer
General description
Immunogen
Preparation Note
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保管分類
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
資料
High titer lentiviral particles including beta-actin, alpha-tubulin and vimentin used for live cell analysis of cytoskeleton structure proteins.
The field of proteomics is continually looking for new ways to investigate protein dynamics within complex biological samples. Recently, many researchers have begun to use RNA interference (RNAi) as a method of manipulating protein levels within their samples, but the ability to accurately determine these protein amounts remains a challenge. Fortunately, over the past decade, the field of proteomics has witnessed significant advances in the area of mass spectrometry. These advances, both in instrumentation and methodology, are providing researchers with sensitive assays for both identification and quantification of proteins within complex samples. This discussion will highlight some of these methodologies, namely the use of Multiple Reaction Monitoring (MRM) and Protein-AQUA.
関連コンテンツ
Find protein research tools to prepare, isolate, and analyze proteins. Organized by how to extract, protect, purify, enrich, modify, and quantify proteins.
ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.
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