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Merck

A2981

抗ATG4B ウサギ宿主抗体

IgG fraction of antiserum, buffered aqueous solution

別名:

Anti-APG4B, Anti-AUT-like 1 cysteine endopeptidase, Anti-AUTL1, Anti-Autophagin-1, Anti-Autophagy-related cysteine endopeptidase 1, Anti-Autophagy-related protein 4 homolog B, Anti-Cysteine protease ATG4B, Anti-KIAA0943, Anti-hAPG4B

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この商品について

UNSPSC Code:
12352203
NACRES:
NA.41
MDL number:
MFCD09753846
Conjugate:
unconjugated
Clone:
polyclonal
Application:
immunohistochemistry (formalin-fixed, paraffin-embedded sections)
western blot
western blot
Species reactivity:
mouse, rat, human
Citations:
31
Technique(s):
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50-1:100 using biotin/ExtaAvidin®-Peroxidase staining of heat-retrieved human cerebellum sections
western blot: 1:125-1:500 using whole extract of 3T3 cells
western blot: 1:500-1:1,000 using whole extract of rat brain
Uniprot accession no.:
Q9Y4P1

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製品名

抗ATG4B ウサギ宿主抗体, IgG fraction of antiserum, buffered aqueous solution

biological source

rabbit

conjugate

unconjugated

antibody form

IgG fraction of antiserum

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen ~44 kDa

species reactivity

mouse, rat, human

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50-1:100 using biotin/ExtaAvidin®-Peroxidase staining of heat-retrieved human cerebellum sections
western blot: 1:125-1:500 using whole extract of 3T3 cells
western blot: 1:500-1:1,000 using whole extract of rat brain

UniProt accession no.

Q9Y4P1

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Quality Level

200

Gene Information

human ... ATG4B(23192)
mouse ... Atg4b(66615)
rat ... Atg4b(316640)

Application

Anti-ATG4B antibody produced in rabbit has been used in
  • western blotting
  • immunohistochemistry

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunohistochemistry (1 paper)
Western Blotting (1 paper)

Biochem/physiol Actions

ATG4B (autophagy-related 4B cysteine peptidase) participates in the modulation of autophagy in mammalian cells. It is also involved in light chain 3 (LC3) lipidation. ATG4B is responsible for the processing of the mammalian Atg8 homologues: Golgi-associated ATPase enhancer of 16 kDa (GATE16), gamma-aminobutyric-acid-type-A-receptor-associated protein (GABARAP), and LC3. It removes the carboxyl-terminal region of proLC3 immediately after its synthesis, generating the soluble LC3-I and exposing a carboxyl-terminal Gly.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

ATG4B (autophagy-related 4B cysteine peptidase), a mammalian orthologue of yeast Atg4, is a cysteine protease. It is widely expressed in human and rat tissues, being most abundant in the brain. This gene is mapped to human chromosome 2q37.
ATG4B is a member of the C-54 family of cysteine proteases that is involved in cell degradation through autophagy.

Immunogen

synthetic peptide corresponding to amino acids 6-22 of human ATG4B, conjugated to KLH via a C-terminal cysteine residue. The corresponding sequence is identical in mouse.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Legal Information

ExtrAvidin is a registered trademark of Merck KGaA, Darmstadt, Germany

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保管分類

10 - Combustible liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

適用法令

試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。

A2981-200UL: + A2981-25UL: + A2981-BULK: + A2981-VAR:

jan

最新バージョンのいずれかを選択してください：

試験成績書（COA）

Lot/Batch Number
089M4802V
097M4773V
045M4855V
042M4777V
051M4807

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以前この製品を購入いただいたことがある場合

文書ライブラリで、最近購入した製品の文書を検索できます。

文書ライブラリにアクセスする

Let-7i-Induced Atg4B Suppression Is Essential for Autophagy of Placental Trophoblast in Preeclampsia
Xu Y, et al.
Journal of Cellular Physiology, 232(9), 2581-2589 (2017)
Essential role for the ATG4B protease and autophagy in bleomycin-induced pulmonary fibrosis
Cabrera S, et al.
Autophagy, 11(4), 670-684 (2015)
Identification of breast cancer cell subtypes sensitive to ATG4B inhibition
Bortnik S, et al.
Testing, 7(41), 66970-66970 (2016)
AKT-mediated phosphorylation of ATG4B impairs mitochondrial activity and enhances the Warburg effect in hepatocellular carcinoma cells
Ni Z, et al.
Autophagy, 14(4), 685-701 (2018)
The 2q37-deletion syndrome: an update of the clinical spectrum including overweight, brachydactyly and behavioural features in 14 new patients
Leroy C, et al.
European Journal of Human Genetics, 21(6), 602-602 (2013)
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