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この商品について
Conjugate:
alkaline phosphatase conjugate
Clone:
polyclonal
Application:
direct ELISA
immunohistochemistry (formalin-fixed, paraffin-embedded sections)
western blot
immunohistochemistry (formalin-fixed, paraffin-embedded sections)
western blot
Species reactivity:
goat
Citations:
66
Technique(s):
direct ELISA: 1:30,000
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50
western blot: 1:30,000
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50
western blot: 1:30,000
製品名
抗ヤギIgG (全分子)-アルカリフォスファターゼ ウサギ宿主抗体, affinity isolated antibody, buffered aqueous glycerol solution
biological source
rabbit
conjugate
alkaline phosphatase conjugate
antibody form
affinity isolated antibody
antibody product type
secondary antibodies
clone
polyclonal
form
buffered aqueous glycerol solution
species reactivity
goat
technique(s)
direct ELISA: 1:30,000
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50
western blot: 1:30,000
shipped in
wet ice
storage temp.
2-8°C
target post-translational modification
unmodified
Quality Level
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関連するカテゴリー
Biochem/physiol Actions
全てのヤギイムノグロブリンに結合します。
Physical form
0.05M Trisバッファ-溶液 (pH 8.0, 1mM MgCl2, 10mM グリシン, 1%BSA, 50%グリセロ-ル, 15mM アジ化ナトリウム含有)
Application
Anti-Goat IgG (whole molecule)-Alkaline Phosphatase antibody is suitable for use in immunoblotting. The product can also be used for direct ELISA (1:30,000) and immunohistochemistry (1:50 using formalin-fixed, paraffin-embedded sections).
Detection of TLR2 and TLR4 in protein extracts from epidermal keratinocytes was performed by western blot using alkaline phosphatase conjugated rabbit anti-goat IgG as the secondary at a 1:2500 dilution.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description
IgG antibodies regulate several functions such as complement activation and phagocytosis. Thus they play a crucial role in facilitating cytological immune responses. Polyclonal anti-goat IgG (whole molecule)–alkaline phosphatase antibody (diluted 1:15,000) can be used as a secondary antibody for immunoblotting of Sall4. This antibody can also be used in immunohistochemistry (diluted1:400) . Rabbit anti-goat IgG antibody reacts specifically with goat IgG and normal goat serum.
Primary goat antibodies are often used to study target proteins for various clinical and research purposes. Thus, secondary anti-goat antibody conjugated to a detectable substrate can be used to facilitate the accurate detection and localization of target proteins.
Immunogen
Purified goat IgG.
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保管分類
10 - Combustible liquids
wgk
WGK 2
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
A4187-.05ML: + A4187-VAR: + A4187-BULK: + A4187-.5ML: + A4187-.25ML: + A4187-1ML:
jan
C Fajardo-Lira et al.
Journal of dairy science, 83(10), 2190-2199 (2000-10-26)
In the present study, we investigated the effect of Pseudomonas spp. growth on the plasmin enzymatic system in casein and whey fractions of fresh milk. Two bacterial strains, Pseudomonas spp. SRM28A and Pseudomonas fluorescens M3/6, were inoculated at a level
Andor Pivarcsi et al.
International immunology, 15(6), 721-730 (2003-05-17)
Keratinocytes have the ability to kill pathogenic fungi and bacteria by producing antimicrobial substances. Recent studies suggest that microbial components use signaling molecules of the human Toll-like receptor (TLR) family to transduce signals in various cells. Here we provide evidence
Menhaj et al.
Planta, 209(4), 406-413 (1999-11-07)
An antibody was raised against the protein HL#2 which is a nuclear-encoded light-stress-induced protein of barley (Hordeum vulgare L.). The expression of the mRNA and the protein of HL#2 was determined under the influence of high light and methyl jasmonate.
L Moysset et al.
Planta, 213(4), 565-574 (2001-09-15)
The intracellular localization of phytochrome in the pulvini of Robinia pseudoacacia L. was analyzed by immunogold electron microscopy after red (R; 15 min) and far-red (FR; 5 min) irradiation 2 h after the beginning of the photoperiod. Screening of the
Gregory G Martin et al.
Archives of biochemistry and biophysics, 588, 25-32 (2015-11-07)
Both sterol carrier protein-2/sterol carrier protein-x (SCP-2/SCP-x) and liver fatty acid binding protein (L-FABP) have been proposed to function in hepatobiliary bile acid metabolism/accumulation. To begin to address this issue, the impact of ablating L-FABP (LKO) or SCP-2/SCP-x (DKO) individually
ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.
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