A8275
抗ウサギIgG (全分子)-ペルオキシダーゼ ヤギ宿主抗体
IgG fraction of antiserum, buffered aqueous solution
別名:
Goat Anti-Rabbit IgG (whole molecule)–HRP
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この商品について
Conjugate:
peroxidase conjugate
Clone:
polyclonal
Application:
ELISA (d)
Citations:
73
biological source
goat
conjugate
peroxidase conjugate
antibody form
IgG fraction of antiserum
antibody product type
secondary antibodies
clone
polyclonal
form
buffered aqueous solution
technique(s)
direct ELISA: 1:10,000
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
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General description
IgG, a monomer, is an antibody circulating in blood and can assist in phagocytic destruction of foreign microorganism. It plays an important role in Antibody dependent cell mediated cytotoxicity and is also associated with type II and type III hypersensitivity. Anti-rabbit IgG (whole molecule) peroxidase conjugate antibody can be used in pAb-ELISA. Rabbit anti-IgG antibody coupled with peroxidase (1:2500 dilution in TBS, 0.5% BSA) can be used in western blots. This antibody is also used as detector in indirect ELISA for saxitoxin analysis. Goat anti-rabbit IgG Peroxidase conjugate reacted specifically with all rabbit immunoglobulin.
Immunogen
Purified rabbit IgG.
Application
二次抗体としてHRP抱合ヤギ抗ウサギIgGを用い、C33A細胞溶解物の共免疫沈降分析およびウエスタンブロット分析が行われました。
Anti-rabbit IgG (whole molecule) peroxidase conjugate antibody is used in western blotting, Indirect ELISA, Immunoblotting and Immunodetection.
Immunohistochemistry was performed on frozen sections (10um) of mouse intestine, liver, and spleen using HRP-conjugated goat anti-rabbit IgG as the secondary antibody. Prior to incubation with the secondary, sections were treated with a mixture of MeOH/hydrogen peroxide 30% to block endogenouse peroxidases.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4 containing 1% bovine serum albumin with preservative.
Preparation Note
Wilson, M.B., and Nakane, P.K., Immunofluorescence and Related Staining Techniques, Elsevier/North Holland Biomedical Press, Amsterdam, p215 (1978) に示された過ヨウ素酸塩法を用いて調製しました。
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Warning
hcodes
Hazard Classifications
Aquatic Chronic 2 - Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1
保管分類
12 - Non Combustible Liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
A8275-2ML:4548173275833 + A8275PROC: + A8275-1ML:4548173187396 + A8275-VAR: + A8275-.5ML:4548173187389 + A8275-BULK:
jan
Immunoblotting and immunodetection.
Gallagher S, Winston SE, Fuller SA
Current Protocols in Immunology, doi: 10-doi: 10 (2001)
Sacha A Jensen et al.
Proceedings of the National Academy of Sciences of the United States of America, 111(28), 10155-10160 (2014-07-02)
Fibrillin microfibrils are 10-12 nm diameter, extracellular matrix assemblies that provide dynamic tissues of metazoan species with many of their biomechanical properties as well as sequestering growth factors and cytokines. Assembly of fibrillin monomers into microfibrils is thought to occur
Arati Limaye et al.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 20(7), 959-961 (2006-04-11)
Oral delivery of biopharmaceutical proteins expressed in plant cells should reduce their cost of production, purification, processing, cold storage, transportation, and delivery. However, poor intestinal absorption of intact proteins is a major challenge. To overcome this limitation, we investigate here
Qinyi Zhu et al.
Oncology letters, 13(6), 4302-4306 (2017-06-11)
Epithelial ovarian cancer (EOC) is one of the main causes of cancer-associated mortality in females with gynecological malignancies. Duffy antigen receptor for chemokines (DARC) has previously been reported to be involved in tumor growth and the inhibition of tumor metastasis.
Anton A Komar et al.
The EMBO journal, 22(5), 1199-1209 (2003-02-28)
The [URE3] phenotype in Saccharomyces cerevisiae is caused by the inactive, altered (prion) form of the Ure2 protein (Ure2p), a regulator of nitrogen catabolism. Ure2p has two functional domains: an N-terminal domain necessary and sufficient for prion propagation and a
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