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この商品について
Conjugate:
unconjugated
Clone:
polyclonal
Application:
immunoelectrophoresis
indirect ELISA
indirect ELISA
Species reactivity:
yeast
Citations:
125
Technique(s):
immunoelectrophoresis: suitable
indirect ELISA: 1:15,000-1:30,000
indirect ELISA: 1:15,000-1:30,000
製品名
抗グルコース-6-リン酸デヒドロゲナーゼ(G-6-PDH)抗体 ウサギ宿主抗体, IgG fraction of antiserum, lyophilized powder
biological source
rabbit
conjugate
unconjugated
antibody form
IgG fraction of antiserum
antibody product type
primary antibodies
clone
polyclonal
form
lyophilized powder
species reactivity
yeast
technique(s)
immunoelectrophoresis: suitable
indirect ELISA: 1:15,000-1:30,000
shipped in
ambient
storage temp.
2-8°C
target post-translational modification
unmodified
Quality Level
General description
この製品は、免疫電気泳動でG-6-PDHに対する特異性が確認されています。未精製のパン酵母エキス中に含まれる他のタンパク質との反応は観察されていません。
G-6-PDH catalyzes the NADP+(or NAD+)-dependent oxidation of D-glucose-6-phosphate to 6-phospho-D-glucono-1,5-lactone. This reaction is the rate limiting step of the pentose phosphate pathway.
Yeast G-6-PDH is often used as a loading control in several immunoassays.
Yeast G-6-PDH is often used as a loading control in several immunoassays.
Glucose-6-phosphate dehydrogenase (G6PD) is a cytosolic enzyme. It synthesizes NADPH (nicotinamide adenine dinucleotide phosphate). The G6PD gene is located on human chromosome X. It spans 18.5 kb and has 13 exons.
Immunogen
パン酵母(S. cerevisiae)由来G-6-PDH 。
Physical form
凍結乾燥品 (0.01M PBS溶液)。
Preparation Note
脱イオン水 2mLで復元して下さい。
Application
Anti-Glucose-6-Phosphate Dehydrogenase (G-6-PDH) antibody has been used to detect the immunoreactive mass of glyceraldehyde 3-phosphate dehydrogenase (GAPDH). It has also been used in western blotting.
Rabbit Anti-Glucose-6-Phosphate Dehydrogenase (G-6-PDH) antibody has been used for western blot assays. The antibody can also be used for indirect ELISA (1:15,000-1:30,000) and immunoelectrophoresis.
Yeast cell extracts were analyzed by western blot and equal loading was determined by probing with rabbit anti-Glucose-6-Phosphate Dehydrogenase antibody.
Biochem/physiol Actions
NADPH (nicotinamide adenine dinucleotide phosphate), produced by glucose-6-phosphate dehydrogenase (G6PD) is an electron donor molecule, that helps in neutralizing harmful oxidizing agents. Deficiency in G6PD shows symptoms, that includes neonatal jaundice and acute episodic hemolysis. This enzyme guards the red blood cells against oxidative challenges.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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保管分類
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
A9521-VAR: + A9521-BULK: + A9521-1VL: + A9521-1VL-PW:
jan
Molecular Hematopathology
Hematologic Pathology, 712-760 (2019)
A conserved C-terminal element in the yeast Doa10 and human MARCH6 ubiquitin ligases required for selective substrate degradation
Zattas D, et al.
The Journal of Biological Chemistry, 29(7), jbc-M116 (2016)
Cytochemical flow analysis of intracellular G6 PD and aggregate analysis of mosaic G6 PD expression
Kalnoky M, et al.
European Journal of Haematology, 100(3), 294-303 (2018)
Danazol inhibits aromatase activity of endometriosis-derived stromal cells by a competitive mechanism
Murakami K, et al.
Fertility and Sterility, 86(2), 291-297 (2006)
Nadya Morozova et al.
Nature cell biology, 8(11), 1263-1269 (2006-10-17)
Ypt-Rab GTPases are key regulators of the various steps of intracellular trafficking. Guanine nucleotide-exchange factors (GEFs) regulate the conversion of Ypt-Rabs to the GTP-bound state, in which they interact with effectors that mediate all the known aspects of vesicular transport.
ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.
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