C3138
カテプシンD from bovine spleen
lyophilized powder, ≥2.0 units/mg protein
別名:
CTSD
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この商品について
CAS番号:
UNSPSC Code:
12352204
NACRES:
NA.32
MDL number:
Specific activity:
≥2.0 units/mg protein
Assay:
10—70% protein (biuret)
Biological source:
bovine spleen
biological source
bovine spleen
Quality Level
assay
10—70% protein (biuret)
form
lyophilized powder
specific activity
≥2.0 units/mg protein
mol wt
~45 kDa
manufacturer/tradename
Sigma-Aldrich
technique(s)
activity assay: suitable
color
dark brown
suitability
suitable for molecular biology
UniProt accession no.
application(s)
life science and biopharma
storage temp.
−20°C
Gene Information
cow ... CTSD(282883)
General description
Research area: Cell Signaling
Cathepsin D is a soluble endosomal-lysosomal aspartic protease and is synthesized in the rough endoplasmic reticulum as preprocathepsin D. It is encoded by the CTSD gene located in the 11p15.5 region.
Cathepsin D is a soluble endosomal-lysosomal aspartic protease and is synthesized in the rough endoplasmic reticulum as preprocathepsin D. It is encoded by the CTSD gene located in the 11p15.5 region.
Application
Cathepsin D from bovine spleen has been used:
- in in vitro dose-dependent fluorometric activity assays.
- in in vitro myelin oligodendrocyte glycoprotein (MOG) digestion to study the uptake of malondialdehyde (MDA)-modified MOG and its implications in central nervous system autoimmunity.
- for enzymatic digestion of the proteoglycan moiety of the articular cartilage in order to determine its dynamic elastic modulus at two different levels of tissue organization.
- in cathepsin D activity assay.
Biochem/physiol Actions
エンドソ-ム-リソソ-ムのアスパラギン酸プロテア-ゼであり、乳がん転移やアルツハイマ-病に関与しています。ヒト包皮繊維芽細胞のアポト-シスにおいて、カテプシンDのリソソ-ム放出はシトクロムcの遊離や膜電位の低下よりも前に起こることが確認されています。
カテプシンDはエンドソ-ム-リソソ-ムのアスパラギン酸プロテア-ゼであり、乳がん転移やアルツハイマ-病に関与しています。ヒト包皮繊維芽細胞のアポト-シスにおいて、カテプシンDのリソソ-ム放出はシトクロムcの遊離や膜電位の低下よりも先に起こるということが確認されています。PC12細胞のカテプシンD量は、アポト-シス誘導後12~24時間で増加します。
Physical form
凍結乾燥品 (クエン酸バッファ-塩含有)。
Other Notes
1ユニットは、基質としてヘモグロビンを使用し、pH 3.0、37°Cで1分あたりTCA可溶性生成物のA280値を1.0増加させる量です(光路長1 cm)。
保管分類
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
C3138-VAR: + C3138-5UN: + C3138-BULK: + C3138-40UN: + C3138-20UN: + C3138-10UN:
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ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.
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