コレステロールエステラーゼ ウシ膵臓由来

タンパク質はBiuret法で測定。

多くのコレステロ-ルの脂肪酸エステルの加水分解に関与する酵素です。
至適pH:6~8
活性化剤:ビスフェノ-ルAジグリシジルエ-テル, cAMP依存性プロテインキナ-ゼII, エタノ-ル, メタノ-ル, n-ブタノ-ル, ホスファチジルコリン, ホスファチジルエタノ-ルアミン, タウロコ-ル酸ナトリウム
阻害剤:ビスフェノ-ルAメタクリル酸, ジイソプロピルフルオロリン酸, エノラ-ゼ, Hg²⁺, フッ化ナトリウム, ホスファチジン酸, ホスファチジルコリン, ホスファチジルセリン

Cholesterol Esterase from bovine pancreas has been used:

• in γ-oryzanol-hydrolyzing activity
• in cholesterol esterase enzyme activity for quantification of cholesterol and cholesterol esters associated in tear film and lenses samples
• to test in vitro degradation of poly(fumaroyl bioxirane) maleate (PFM) polymer designed for bone tissue engineering

Cholesterol esterase bound to membrane-associated heparin on brush border membranes aids in the transport of cholesterol and free fatty acid across the membrane. This enzyme is widely used in the determination of serum cholesterol in clinical laboratories. The enzyme from Sigma has been used to evaluate the inhibitory and antioxidant functions of the methanol extract of the Camellia sinensis leaves under in vitro conditions. The enzyme has also been used to digest human serum samples to confirm the presence and position of acyl esters of 7α-hydroxycholesterol.

1ユニットは、タウロコール酸存在下、pH 7.0、37°C、1分間で、1.0 μmolのオレイン酸コレステロールをコレステロールとオレイン酸に加水分解する酵素量です。

部分精製済み

Cholesterol esterase (CE) is a reversible enzyme that can hydrolyze or synthesize fatty acid esters of cholesterol and other sterols. Hydrolysis of water insoluble long chain fatty acid esters requires bile salt activation. Hydrolysis of water soluble esters of short chain fatty acids and lysophospholipids does not require activation by bile salts. It also hydrolyzes tri-, di-, and mono-acylglycerols, phospholipids, lysophospholipids, and ceramide. This monomeric glycoprotein may have multiple functions in lipid and lipoprotein metabolism, as well as in atherosclerosis. Its molecular mass is found to be 84 kDa.

Cholesterol Esterase from bovine pancreas is a member of α-β hydrolase superfamily. The core of the protein comprises 11 β-strands, surrounded by 15 α-strands. Catalytic triad is in the centre of the molecule. The C-terminus possesses hydrophobic residues.

This product is partially purified from bovine pancreas and is supplied as a white to light brown lyophilized powder containing ≥20% protein (biuret) and potassium phosphate.

Cholesterol esterase is soluble in 0.4 M potassium phosphate, pH 7.0 at 1 mg/mL concentration.