製品名
チオレドキシンレダクターゼアッセイキット, 1 kit sufficient for 100 assays (1 mL)
usage
kit sufficient for 100 assays (1 mL)
shipped in
dry ice
storage temp.
−20°C
Quality Level
Gene Information
human ... TXNRD1(7296), TXNRD2(10587), TXNRD3(114112)
関連するカテゴリー
Application
ラット肝臓の核除去抽出物を用いた場合、本キットでは0.25 unit/mLまで直線性を示します。このサンプルの場合、活性の約20%は非特異的な還元酵素の活性(TrxRインヒビタ-耐性)によるものです。
Features and Benefits
- 簡便な方法です。
- 本キットには、哺乳類のチオレドキシンレダクタ-ゼの比色定量に必要な試薬が全て揃っています。
- キットには哺乳類チオレドキシンレダクタ-ゼを特異的に阻害するインヒビタ-溶液も準備されています。
- キットはHeLa細胞、A549細胞、ジャ-カット細胞、U937細胞、A431細胞、COS細胞、CHO細胞、NIH 3T3細胞などの細胞株由来ライセ-トや心筋で試験されています。
General description
チオレドキシンレダクターゼアッセイキットは、哺乳類の細胞および組織中のチオレドキシンレダクターゼ活性を比色定量で決定します。5,5'-ジチオビス(2-ニトロ安息香酸)(DTNB)とNADPHの還元反応に基づいており、反応で生成する濃黄色の5-チオ-2-ニトロ安息香酸(TNB)を412 nmで測定します。
DTNB + NADPH + H+ ↔ 2 TNB + NADP+
DTNB + NADPH + H+ ↔ 2 TNB + NADP+
キットの構成要素のみ
製品番号
詳細
- Assay Buffer 5x for Thioredoxin Reductase 30 mL
- Thioredoxin Reductase 10 μg
- Thioredoxin Reductase Inhibitor Solution .05 mL
- 5,5'-Dithiobis(2-nitrobenzoic) acid (DNTB) 150 mg
- NADPH 25 mg
- Dimethyl Sulfoxide (DMSO) 7.5 mL
signalword
Warning
hcodes
Hazard Classifications
Repr. 2
保管分類
10 - Combustible liquids
flash_point_f
188.6 °F
flash_point_c
87 °C
Matthew T Harper
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Au(NHC) and Au(NHC)2, i.e. a monocarbene gold(I) complex and the corresponding bis(carbene) complex, are two structurally related compounds, endowed with cytotoxic properties against several cancer cell lines. Herein, we explore the molecular and cellular mechanisms at the basis of their
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This study investigated the effects of incubation period and melatonin treatment on red blood cell (RBC) metabolism in an auto-incubation model of H2O2-induced oxidative stress. The study was carried out on three healthy adult donors by incubating RBCs in their
ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.
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