D1152
ダルベッコ改変イーグル培地(高グルコース)
HEPES Modification, With 4500 mg/L glucose, L-glutamine, and 25 mM HEPES, without sodium bicarbonate and pyruvate, powder, suitable for cell culture
別名:
DME, DMEM
製品名
ダルベッコ改変イーグル培地(高グルコース), HEPES Modification, With 4500 mg/L glucose, L-glutamine, and 25 mM HEPES, without sodium bicarbonate and pyruvate, powder, suitable for cell culture
form
powder
technique(s)
cell culture | mammalian: suitable
components
sodium pyruvate: no
NaHCO3: no
phenol red: yes
HEPES: 25 mM
L-glutamine: yes
glucose: high
shipped in
ambient
storage temp.
2-8°C
Quality Level
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関連するカテゴリー
Application
ダルベッコ改変イーグル培地(DMEM)は、イーグル基礎培地(BME)を改変したもので、4倍濃度のアミノ酸とビタミンが含まれています。オリジナルの処方には、グルコース1000 mg/Lが含まれ、胚性マウス細胞の培養に使用されていました。それ以降、マウス、ニワトリの細胞のほか、様々な正常細胞やトランスフォーム細胞の初代培養をサポートするために幾通りかの方法で改変が行われてきました。これらの培地は、L-グルタミンとピルビン酸ナトリウムの組み合わせがそれぞれに異なります。また、グルコース濃度が4500 mg/Lまで引き上げられたことから、「DMEM/High」と命名されました。
Preparation Note
17.3 gの粉末で1 Lの培地ができる配合です。
3.7 g/Lの炭酸水素ナトリウムを添加してください。
保管分類
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Farlen J B Miranda et al.
Poultry science, 95(1), 12-18 (2016-01-23)
Raising ostriches became an important economic activity after their products became commodities. The health of farm animals is of paramount importance, so assessing basic immunological responses is necessary to better understand health problems. We developed a method to obtain ostrich
Kiran Pandey et al.
Animal reproduction science, 181, 93-102 (2017-04-24)
Gonadotropin-releasing hormone receptors (GnRHRs) colocalize with insulin and glucocorticoid receptors in lipid rafts of the gonadotroph plasma membrane, where they facilitate downstream signaling. We recently found that orphan G-protein-coupled receptor (GPR)61 is expressed in the anterior pituitary (AP) of heifers
Kiran Pandey et al.
Animal science journal = Nihon chikusan Gakkaiho, 89(1), 60-71 (2017-09-30)
We recently found that orphan G-protein-coupled receptor (GPR)153 is expressed in the anterior pituitary (AP) of heifers, leading us to speculate that GPR153 colocalizes with gonadotropin-releasing hormone receptor (GnRHR) in the plasma membrane of gonadotrophs and is expressed at specific
Jiping Sun et al.
Journal of cellular and molecular medicine, 22(10), 4840-4855 (2018-07-20)
BMSCs are important in replacement therapy of diabetic nephropathy (DN). MiR-124a exerts effect on the differentiation capability of pancreatic progenitor cells. The objective of this study was to explore the molecular mechanisms, the functions of miR-124a and bone marrow mesenchymal
Christopher A Drummond et al.
Physiological genomics, 48(3), 220-229 (2015-12-25)
Chronic kidney disease (CKD) is accompanied by cardiac fibrosis, hypertrophy, and dysfunction, which are commonly referred to as uremic cardiomyopathy. Our previous studies found that Na/K-ATPase ligands or 5/6th partial nephrectomy (PNx) induces cardiac fibrosis in rats and mice. The
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Instructions
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