製品名
プラスミドDNA 大腸菌RRI由来, pUC19, buffered aqueous solution
SMILES string
[P](=O)(OC2C(OC(C2)N)CO[P](=O)(OC3C(OC(C3)N)CO)O)(OCC1OC(CC1O)N)O
InChI
1S/C15H31N3O13P2/c16-13-1-7(20)11(28-13)5-25-32(21,22)31-9-3-15(18)29-12(9)6-26-33(23,24)30-8-2-14(17)27-10(8)4-19/h7-15,19-20H,1-6,16-18H2,(H,21,22)(H,23,24)
InChI key
AWBASQCACWFTGD-UHFFFAOYSA-N
grade
Molecular Biology
form
buffered aqueous solution
shipped in
dry ice
storage temp.
−20°C
Quality Level
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Biochem/physiol Actions
これらのプラスミドは適合する宿主株に、アンピシリン耐性、β-ガラクトシダ-ゼ相補欠損を付与します。マルチクロ-ニングサイト(MCS)はβ-ガラクトシダ-ゼ遺伝子内に存在します。pUC8, 9は9ヶ所、pUC18, 19は13ヶ所の特異部位をMCS内に有しています。
外来DNAをMCSに挿入することで、乳糖分解能が失われます。プラスミドを含む宿主株は乳糖陽性・アンピシリン耐性で、アンピシリンとX-Galを含むプレ-ト上に青色コロニ-を形成します。一方、MCS内に外来DNAを有するプラスミドを含む宿主株は乳糖陰性・アンピシリン耐性で、培地上に白色のコロニ-を形成します。pUCプラスミドのMCS領域の方向は対応するM13ファ-ジのと同様です。
外来DNAをMCSに挿入することで、乳糖分解能が失われます。プラスミドを含む宿主株は乳糖陽性・アンピシリン耐性で、アンピシリンとX-Galを含むプレ-ト上に青色コロニ-を形成します。一方、MCS内に外来DNAを有するプラスミドを含む宿主株は乳糖陰性・アンピシリン耐性で、培地上に白色のコロニ-を形成します。pUCプラスミドのMCS領域の方向は対応するM13ファ-ジのと同様です。
Foreign DNA inserted at the MCS interrupts the β-galactosidase gene and abolishes the ability to catabolize lactose. Lactose-positive, ampicillin-resistant colonies (host strain containing plasmid) form blue colonies on plates containing ampicillin and X-Gal; lactose-negative, ampicillin-resistant colonies (host strain containing plasmid with foreign DNA inserted at the MCS) form white colonies on this medium.
Application
Plasmid DNA from Escherichia coli RRI has been used for imaging of DNA (nanostructure) via atomic force microscopy.
General description
The pUC19 plasmid (2,686 bp) confers ampicillin resistance and complement defects in β-galactosidase in appropriate host strains. The multiple cloning site (MCS) is within the B-galactosidase gene; the plasmid has thirteen unique sites in the MCS (Acc I, BamH I, EcoR I, Hinc II, Hind III, Kpn I, Pst I, Sac I, Sal I, Sma I, Sph I, Xba I, and Xma I).
Other Notes
pUC19 Plasmid DNA, 10 μg is supplied at approximately 0.5 mg/ml in 10 mM Tris-HCl (pH 8.0) with 1 mM EDTA.
保管分類
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Atomic force microscopy with inherent
disturbance suppression for nanostructure
imaging
disturbance suppression for nanostructure
imaging
A W Sparks
Nanotechnology (2006)
Measurement of DNA Morphological Parameters at Highly Entangled Regime on Surfaces
Annalisa Calo
The Journal of Physical Chemistry (2009)
Who owns human genes?: Is DNA patentable?
Lawrence O Gostin
JAMA, 310(8), 791-792 (2013-07-24)
Jong Bum Lee et al.
Journal of biomedical nanotechnology, 9(7), 1245-1249 (2013-08-06)
Recently, DNA has been used to guide the self-assembly of functional materials. Based on programmability of DNA, branched DNA nanostructures were created and precisely labeled with quantum dots and gold nanoparticles. The precise molecular recognition of DNA allows the precise
Chao Liang et al.
Journal of nanoscience and nanotechnology, 13(6), 3999-4005 (2013-07-19)
LAMP is an isothermal amplification method that can achieve ultra-high sensitivity and specificity. However, the conventional detection of LAMP amplicons can lead to cross-contamination due to the need to open the reaction tube which contains a large number of amplicons.
プロトコル
Preparation for biodegradable nanoparticles and their use in transfection protocols .
関連コンテンツ
Datasheet
ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.
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