D8273
デオキシリボ核酸−セルロース, 一本鎖 仔ウシ胸腺DNA由来
lyophilized powder
別名:
DNA-セルロース
製品名
デオキシリボ核酸−セルロース, 一本鎖 仔ウシ胸腺DNA由来, lyophilized powder
biological source
bovine (calf) thymus
form
lyophilized powder
extent of labeling
≥3 mg single-stranded calf thymus DNA/g
storage temp.
−20°C
Quality Level
関連するカテゴリー
Analysis Note
微量のTrisとEDTAを含有する場合があります。
Application
Deoxyribonucleic acid−cellulose single-stranded from calf thymus DNA is used to study the binding parameters and binding specificity of a variety of proteins, nucleotides, and chemical reagents including anti-cancer agents. It is frequently used in pull-down assays and chromatographic procedures.
General description
Deoxyribonucleic acid−cellulose single-stranded from calf thymus DNA, also known as ctDNA is a natural source of DNA designed for DNA binding agent studies. The detection limits of ctDNA are around 2.0ng and linear ranges of light scattering intensity (I(LS)) is 0.05-1.5 mµg.
保管分類
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
S S Zhang et al.
The Journal of biological chemistry, 266(30), 20483-20490 (1991-10-25)
Two DNA helicases from calf thymus nuclei have been purified and characterized. The two proteins, designated as nuclear DNA helicase I and II, were copurified on Bio-Rex 70, DEAE-Sepharose, phosphocellulose and subsequently separated from each other on a heparin-Sepharose column.
A Georgaki et al.
Nucleic acids research, 22(7), 1128-1134 (1994-04-11)
A DNA helicase from calf thymus, called DNA helicase F, copurified with replication protein A through several steps of purification including DEAE-Sephacel, hydroxyapatite and single stranded DNA cellulose. It is finally separated from replication protein A on FPLC Mono Q
T Kuzuhara et al.
Biochemical and biophysical research communications, 362(4), 805-810 (2007-09-04)
Tumor necrosis factor-alpha (TNF-alpha) inducing protein (Tipalpha) is a carcinogenic factor secreted from Helicobacter pylori (H. pylori), mediated through both enhanced expression of TNF-alpha and chemokine genes and activation of nuclear factor-kappaB. Since Tipalpha enters gastric cancer cells, the Tipalpha
D Pisetsky et al.
The Journal of rheumatology, 26(9), 1934-1938 (1999-09-24)
To elucidate the epitope structure to DNA by identifying antigenic determinants on bacterial DNA bound by anti-DNA antibodies from normal human subjects (NHS) and patients with systemic lupus erythematosus (SLE). Sera from NHS and patients with SLE were tested by
Q Hua et al.
Cellular and molecular life sciences : CMLS, 60(2), 413-421 (2003-04-08)
Tau, a major microtubule-associated protein of the neuron, which is known to promote the assembly of and to stabilize microtubules, has also been seen associated with chromatin in neuronal cell lines, but its role in this subcellular compartment is still
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