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Merck

F2043

モノクロナール抗β-チューブリン−FITC マウス宿主抗体

clone TUB 2.1, purified from hybridoma cell culture

別名:

モノクロナール抗β-チューブリン

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この商品について

NACRES:
NA.41
UNSPSC Code:
12352203
Conjugate:
FITC conjugate
Clone:
TUB 2.1, monoclonal
Application:
direct immunofluorescence
Species reactivity:
human, rat, frog, moth, mouse, plant, rabbit, chicken, bovine, wheat, sea urchin, hamster
Citations:
33
Technique(s):
direct immunofluorescence: 1:25 using cultured BHK cells

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製品名

モノクロナール抗β-チューブリン−FITC マウス宿主抗体, clone TUB 2.1, purified from hybridoma cell culture

biological source

mouse

conjugate

FITC conjugate

antibody form

purified from hybridoma cell culture

antibody product type

primary antibodies

clone

TUB 2.1, monoclonal

form

buffered aqueous solution

mol wt

antigen 55 kDa

species reactivity

human, rat, frog, moth, mouse, plant, rabbit, chicken, bovine, wheat, sea urchin, hamster

storage condition

protect from light

technique(s)

direct immunofluorescence: 1:25 using cultured BHK cells

isotype

IgG1

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Quality Level

200

Gene Information

human ... TUBB(203068), TUBB1(81027), TUBB1(81027), TUBB1(81027), TUBB1(81027), TUBB2A(7280), TUBB2A(7280), TUBB2A(7280), TUBB2A(7280), TUBB2C(10383), TUBB2C(10383), TUBB2C(10383), TUBB2C(10383)
mouse ... Tubb1(104068), Tubb1(104068), Tubb1(104068), Tubb1(104068), Tubb2a(22151), Tubb2a(22151), Tubb2a(22151), Tubb2a(22151), Tubb2c(227613), Tubb2c(227613), Tubb2c(227613), Tubb2c(227613), Tubb3(22152)
rat ... Tubb2(29212), Tubb2(29212), Tubb2(29212), Tubb2(29212), Tubb2c(296554), Tubb2c(296554), Tubb2c(296554), Tubb2c(296554)

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Biochem/physiol Actions

抗体はβ-チュ-ブリンの5種類のアイソフォ-ム(β1-β5)をすべて認識します。イムノブロッティングでは、β-チュ-ブリンのC末端部分のβ-Lcおよびβ-Scフラグメントと反応します。抗体は、培養細胞や組織切片のβ-チュ-ブリンの特定に使用することができます。
Mutation in β-Tubulin gene leads to various neuronal migration disorders such as lissencephaly, pachygyria and polymicrogyria malformations. β-1 tubulin, also known as class VI or TUBB1, plays a vital role in platelet production and is considered to be a potential biomarker candidate for immune thrombocytopenia (ITP).

Immunogen

ラット脳由来チュ-ブリン。

Physical form

0.01M PBS溶液(pH 7.4, 1%BSA, 15mMアジ化ナトリウム含有)。

Application

FITC-β tubulin antibody may be used for detection ajnd localization of β-tubulin by direct labeling in a variety of eukaryotic cells ranging from human embryonic kidney cells (HEK293T) to cells of azuki bean epicotyl. by immunohistochemical and immunocytochemical assays.
Monoclonal Anti-β-Tubulin-FITC antibody has been used:
  • in the evaluation of the status of the meiotic spindle and the arrangement of chromosomes
  • in immunofluorescence studies
  • in immunofluorescence microscopy
  • in spindle and chromosome imagery(6)

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

β-Tubulin belongs to the subfamily of tubulins. It has two β-sheets enclosed by α-helices. It also contains an N-terminal domain with the guanine nucleotide-binding region, an intermediate domain with taxol-binding site and a C-terminal domain, which has the binding surface for molecular motor proteins. Human β-tubulin consists of seven isoforms (βI (class I), βII (class II), βIII (class III), βIVa (class IVa), βIVb (class IVb), βV (class V), and βVI (class VI)). TUBB1 (tubulin beta class I) is located on human chromosome 6p21.33.(10)
Tubulin is the major building block of microtubules. This intracellular cylindrical filamentous structure is present in almost all eukaryotic cells. Microtubules function as structural and mobile elements in mitosis, intracellular transport, flagellar movement, and in the cytoskeleton. Tubulin is a heterodimer, which consists of α-tubulin and β-tubulin; both subunits have a molecular weight of 55 kDa and share considerable homology. The most studied tubulins have been isolated from vertebrate brains. The microtubules can be viewed in immunofluorescent microscopy, which enables the observation of the intracellular organization of proteins that are in the form of a supramolecular structure.

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保管分類

12 - Non Combustible Liquids

wgk

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable

適用法令

試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。

F2043-.5ML: + F2043-BULK: + F2043-VAR: + F2043-.2ML:

jan

最新バージョンのいずれかを選択してください：

試験成績書（COA）

Lot/Batch Number
108M4766V
22180627
028M4794V
22171111
066M4788V

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Transient increase in the transcript levels of gamma-tubulin complex genes during reorientation of cortical microtubules by gravity in azuki bean (Vigna angularis) epicotyls
Soga K, et al.
Journal of Plant Research, 121(5), 493-498 (2008)
Abraham L Kierszenbaum
Molecular reproduction and development, 62(1), 1-3 (2002-04-05)
Two microtubule-containing structures are assembled during spermiogenesis: a transient manchette and a stable axoneme. Both structures contain microtubules enriched in posttranslationally modified tubulins. Despite the existence of a spectrum of tubulin isotypes postulated by the multi-tubulin hypothesis, further extended by
Peloruside-and laulimalide-resistant human ovarian carcinoma cells have betaI-tubulin mutations and altered expression of betaII-and betaIII-tubulin isotypes
Kanakkanthara A, et al.
Molecular Cancer Therapeutics, 393(6681), molcanther-molcan1057 (2011)
Changes to the meiotic spindle and zona pellucida of mature mouse oocytes following different cryopreservation methods
Ko C S, et al.
Animal Reproduction Science, 105(3-4), 272-282 (2008)
J C Bruusgaard et al.
Journal of applied physiology (Bethesda, Md. : 1985), 100(6), 2024-2030 (2006-02-25)
We have recently published a new technique for visualizing nuclei in living muscle fibers of intact animals, based on microinjection of labeled DNA into single myofibers, excluding satellite cells (Bruusgaard JC, Liestol K, Ekmark M, Kollstad K, and Gundersen K.
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資料

Antibodies to Tubulins, including Centrosome-Associated ε-Tubulin

Microtubules of the eukaryotic cytoskeleton are composed of a heterodimer of α- and β-tubulin. In addition to α-and β-tubulin, several other tubulins have been identified, bringing the number of distinct tubulin classes to seven.

εチューブリンを含むチューブリンに対する抗体

αチューブリンとβチューブリンのヘテロダイマーで構成される真核細胞の細胞骨格の微小管には、多様な機能を発揮する複数の明確なチューブリンクラスがあります。

関連コンテンツ

ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.

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