FG0100
遊離グリコール定量キット
1 kit sufficient for 1000 reactions
別名:
トリグリセリドおよび遊離グリセロールキットおよび試薬, トリグリセリド・遊離グリセリンキットおよび試薬
Application
Free Glycerol Determination Kitは、共役酵素反応を用いて遊離した内因性グリセロールを測定しますが、初期のリパーゼ加水分解は含みません。グリセロールは、グリセロールキナーゼ(GK)により触媒される反応において、アデノシン-5'-トリホスファターゼ(ATP)によりリン酸化され、グリセロール-1-リン酸(G-1-P)およびアデノシン-5'-二リン酸(ADP)が生成されます。次にG-1-Pが、グリセロールリン酸オキシダーゼ(GPO)により酸化され、ジヒドロキシアセトンリン酸(DAP)および過酸化水素(H2O2)が生成されます。ペルオキシダーゼは、H2O2と4-アミノアンチピリン(4-AAP)および塩化N-エチル-N-(3-スルホプロピル)m-アニシジン(ESPA)との共役を触媒し、キノンイミン染料を生成します。キノンイミン染料は波長540 nmで最大吸光度を示します。波長540 nmにおける吸光度の増加はサンプルのグリセロール濃度に直接比例します。
トリグリセリドキットおよび遊離グリセロールキットは、グリセロール、総トリグリセリドまたは遊離トリグリセリドの定量的測定に使用します。
Suitable for the quantitative enzymatic determination of glycerol in serum or plasma.
The Free Glycerol Determination Kit has been used to determine the level of glycerol released into the medium from mammalian cells grown in vitro .
Other Notes
あまり多くの反応が必要でない場合は、キットではなく、試薬とグリセロ-ル標準物質を個別にご購入いただくことも可能です。
General description
Glycerol, also referred to as glycerin or glycerine, is a 3 carbon sugar alcohol that forms the backbone of fatty acids. It is a central component for synthesis of all lipids, and acts as a backbone for triglycerides and phospholipids, which play an important role in cell membrane structure. Due to its low toxicity, glycerol is widely used in pharmaceutical, food and cosmetic formulations and is the main waste by-product of biodiesel production via transesterification. In addition to kits, the individual reagents and glycerol standard are available separately when fewer reactions are needed.
signalword
Danger
hcodes
Hazard Classifications
Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Skin Irrit. 2
保管分類
10 - Combustible liquids
Hui Gao et al.
EBioMedicine, 30, 248-260 (2018-03-28)
Long non-coding RNAs (lncRNAs) belong to a recently discovered class of molecules proposed to regulate various cellular processes. Here, we systematically analyzed their expression in human subcutaneous white adipose tissue (WAT) and found that a limited set was differentially expressed
Sarah K Trottier et al.
Obesity (Silver Spring, Md.), 25(10), 1707-1715 (2017-08-08)
To compare the individual and combined effects of dairy and endurance exercise training in reducing weight gain and adiposity in a rodent model of diet-induced obesity. An 8-week feeding intervention of a high-fat, high-sugar diet was used to induce obesity
Hong Min Kim et al.
Nutrition (Burbank, Los Angeles County, Calif.), 55-56, 63-70 (2018-07-01)
Non-alcoholic fatty liver disease is characterized by high hepatic triacylglycerol contents, which is associated with endoplasmic reticulum (ER) stress and insulin resistance. Caffeic acid (CA) has antioxidant, immunomodulatory, and antiinflammatory effects. We investigated the effects of CA on hepatic steatosis
Elena Kosenko et al.
Journal of clinical medicine, 9(7) (2020-07-28)
The relationship between liver disease and neuropathology in hepatic encephalopathy is well known, but the genesis of encephalopathy in liver failure is yet to be elucidated. Conceptually, the main cause of hepatic encephalopathy is the accumulation of brain ammonia due
Geraldine Harriman et al.
Proceedings of the National Academy of Sciences of the United States of America, 113(13), E1796-E1805 (2016-03-16)
Simultaneous inhibition of the acetyl-CoA carboxylase (ACC) isozymes ACC1 and ACC2 results in concomitant inhibition of fatty acid synthesis and stimulation of fatty acid oxidation and may favorably affect the morbidity and mortality associated with obesity, diabetes, and fatty liver
資料
Lipid Induced Insulin Resistance
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